Cloned (Comment) | Organism |
---|---|
heterologous production of EctA in Escherichia coli, with either a N-terminal (NH2-WSHPQFEK-SG) or a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide for their purification by affinity chromatography | Paenibacillus lautus |
Crystallization (Comment) | Organism |
---|---|
high-resolution crystal structures at 1.2-2.2 A resolution: 1. for its apo-form, 2. in complex with CoA, 3. in complex with L-2,4-diaminobutanoate, 4. in complex with both CoA and L-2,4-diaminobutanoate, and 5. in the presence of the product (2S)-4-acetamido-2-aminobutanoate | Paenibacillus lautus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 100 mM, 25% loss of activity. 1.5 M, 88% loss of activity | Paenibacillus lautus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-2,4-diaminobutanoate | pH 7.4, 30°C | Paenibacillus lautus | |
2.79 | - |
acetyl-CoA | pH 7.5, 30°C | Paenibacillus lautus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39730 | - |
gel filtration | Paenibacillus lautus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | Paenibacillus lautus | the enzyme catalyzes a step in ectoine biosynthesis | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus lautus | A0A385TZ63 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus lautus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | - |
Paenibacillus lautus | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | the enzyme catalyzes a step in ectoine biosynthesis | Paenibacillus lautus | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20120, enzyme withe a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide, SDS-PAGE | Paenibacillus lautus |
homodimer | 2 * 20550, enzyme with a N-terminal (NH2-WSHPQFEK-SG) Strep-Tag II peptide, SDS-PAGE | Paenibacillus lautus |
Synonyms | Comment | Organism |
---|---|---|
EctA | - |
Paenibacillus lautus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is only active for very short times at temperatures higher than 40 °C | Paenibacillus lautus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Paenibacillus lautus |
General Information | Comment | Organism |
---|---|---|
evolution | crystal structures of the enzyme in complex with the cosubstrate reveals an evolutionarily conserved CoA-binding site | Paenibacillus lautus |
metabolism | the enzyme catalyzes a step in ectoine biosynthesis | Paenibacillus lautus |