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Literature summary for 2.3.1.178 extracted from
- Genes and enzymes of ectoine biosynthesis in halotolerant methanotrophs (2011), Methods Enzymol., 495, 15-30.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Methylotuvimicrobium alcaliphilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Methylophaga alcalica |  |
| Cd2+ | - |
Methylophaga thalassica | |
| Cd2+ | - |
Methylotuvimicrobium alcaliphilum | |
| Cu2+ | - |
Methylophaga alcalica | |
| Cu2+ | - |
Methylophaga thalassica | |
| Zn2+ | - |
Methylophaga alcalica | |
| Zn2+ | - |
Methylophaga thalassica | |
| Zn2+ | - |
Methylotuvimicrobium alcaliphilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
acetyl-CoA | pH 9.5, 25°C | Methylophaga alcalica | |
| 0.037 | - |
acetyl-CoA | pH 9.5, 25°C | Methylotuvimicrobium alcaliphilum | |
| 0.076 | - |
acetyl-CoA | pH 9.5, 25°C | Methylophaga thalassica | |
| 0.365 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylophaga thalassica | |
| 0.375 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylophaga alcalica | |
| 0.465 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylotuvimicrobium alcaliphilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates, best at 0.25 M | Methylotuvimicrobium alcaliphilum | |
| additional information | no activation by NaCl and KCl | Methylophaga thalassica | |
| additional information | no activation by NaCl and KCl | Methylophaga alcalica | |
| NaCl | activates, best at 0.1-0.2 M | Methylotuvimicrobium alcaliphilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga thalassica |
| 20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
| 20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga alcalica |
| 40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylophaga thalassica |
| 40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylotuvimicrobium alcaliphilum |
| 40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylophaga alcalica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga thalassica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | Halomonas elongata | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | Methylotuvimicrobium alcaliphilum | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga alcalica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | Methylotuvimicrobium alcaliphilum 20Z | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halomonas elongata | - |
- |
- |
| Methylophaga alcalica | - |
gene ectA | - |
| Methylophaga thalassica | - |
gene ectA | - |
| Methylotuvimicrobium alcaliphilum | - |
gene ectA | - |
| Methylotuvimicrobium alcaliphilum 20Z | - |
gene ectA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylophaga thalassica |
| 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylotuvimicrobium alcaliphilum |
| 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylophaga alcalica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | - |
Halomonas elongata | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum 20Z | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
| additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylophaga thalassica | ? | - |
? | |
| additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Halomonas elongata | ? | - |
? | |
| additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylotuvimicrobium alcaliphilum | ? | - |
? | |
| additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylophaga alcalica | ? | - |
? | |
| additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga thalassica |
| homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
| homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga alcalica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DABA acetyltransferase | - |
Methylophaga thalassica |
| DABA acetyltransferase | - |
Halomonas elongata |
| DABA acetyltransferase | - |
Methylotuvimicrobium alcaliphilum |
| DABA acetyltransferase | - |
Methylophaga alcalica |
| EctA | - |
Methylophaga thalassica |
| EctA | - |
Halomonas elongata |
| EctA | - |
Methylotuvimicrobium alcaliphilum |
| EctA | - |
Methylophaga alcalica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Methylotuvimicrobium alcaliphilum |
| 25 | - |
assay at | Halomonas elongata |
| 30 | 35 | - |
Methylophaga thalassica |
| 30 | 35 | - |
Methylophaga alcalica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Halomonas elongata |
| 8.5 | - |
- |
Methylophaga thalassica |
| 9.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
| 9.5 | - |
- |
Methylophaga alcalica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylophaga thalassica |
| metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Halomonas elongata |
| metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylotuvimicrobium alcaliphilum |
| metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylophaga alcalica |
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