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Literature summary for 2.3.1.178 extracted from

  • Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.
    Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata (1999), J. Bacteriol., 181, 91-99.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
enzyme is stabilized in presence of NaCl. Residual activity after a 3 h incubation at 0°C is 100% in presence of 2 M NaCl Halomonas elongata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Halomonas elongata

Organism

Organism UniProt Comment Textmining
Halomonas elongata
-
OUT30018
-
Halomonas elongata OUT30018
-
OUT30018
-

Purification (Commentary)

Purification (Comment) Organism
-
Halomonas elongata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-2,4-diaminobutanoate
-
Halomonas elongata CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
Halomonas elongata OUT300318 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
Halomonas elongata OUT30018 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Halomonas elongata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
residual activity after a 3 h incubation at 30°C is 62% in presence of 2 M NaCl Halomonas elongata
37
-
residual activity after a 3 h incubation at 37°C is 35% in presence of 2 M NaCl Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Halomonas elongata