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Literature summary for 2.3.1.177 extracted from

  • Chizzali, C.; Swiddan, A.K.; Abdelaziz, S.; Gaid, M.; Richter, K.; Fischer, T.C.; Liu, B.; Beerhues, L.
    Expression of biphenyl synthase genes and formation of phytoalexin compounds in three fire blight-infected Pyrus communis cultivars (2016), PLoS ONE, 11, e0158713 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.177

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
0.0011
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
0.0116
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
0.0137
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
0.0784
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
0.0952
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
0.326
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
0.389
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42960
-
 calculated from sequence Pyrus communis
43050
-
 calculated from sequence Pyrus communis

Organism

Organism UniProt Comment Textmining
Pyrus communis A0A1B1PJB0 cultivars: Conference, Harrow Sweet and Alexander Lucas
-
Pyrus communis A0A1B1PJB7 cultivars: Conference, Harrow Sweet and Alexander Lucas
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrus communis

Source Tissue

Source Tissue Comment Organism Textmining
leaf expressed in cultivar Harrow Sweet, not in Alexander Lucas and Conference after inoculation with Erwinia amylovora. No expression of the PcBIS1 gene in the healthy leaves Pyrus communis
-
leaf expressed in cultivars Harrow Sweet, Alexander Lucas and Conference after inoculation with Erwinia amylovora. No expression of the PcBIS2 gene in the healthy leaves Pyrus communis
-
stem transition zone of the stem, expressed in cultivar Harrow Sweet, not in Alexander Lucas and Conference after inoculation with Erwinia amylovora Pyrus communis
-
stem transition zone of the stem, expressed in cultivars Harrow Sweet, Alexander Lucas and Conference after inoculation with Erwinia amylovora Pyrus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + benzoyl-CoA benzoyldiacetic acid lactone (6-phenyl-4-hydroxy-2-pyrone) is formed as a minor by-product Pyrus communis 4 CoA + 3,5-dihydroxybiphenyl + 4 CO2
-
 ?
3 malonyl-CoA + salicoyl-CoA benzoyldiacetic acid lactone (6-phenyl-4-hydroxy-2-pyrone) is formed as a minor by-product Pyrus communis 4 CoA + 4-hydroxycoumarin + 4 CO2
-
 ?

Synonyms

Synonyms Comment Organism
PcBIS1
-
 Pyrus communis
PcBIS2
-
 Pyrus communis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pyrus communis
35
-
-
 Pyrus communis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.035
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
0.055
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
0.77
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
0.89
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Pyrus communis
7.5
-
-
 Pyrus communis

Expression

Organism Comment Expression
Pyrus communis the relative transcript levels increases up to 800fold in cultivar Harrow Sweet after Erwinia amylovora inoculation up
Pyrus communis the relative transcript levels increases up to 800fold in cultivar Harrow Sweet after Erwinia amylovora inoculation, in cultivars Conference and Alexander Lucas up to 1000fold up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.3
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
9.8
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
38.9
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
50
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis