BRENDA - Enzyme Database show
show all sequences of 2.3.1.174

Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase

Kaschabek, S.R.; Kuhn, B.; Muller, D.; Schmidt, E.; Reineke, W.; J. Bacteriol. 184, 207-215 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, 90% inhibition
Pseudomonas sp.
additional information
1 mM EDTA or 1,10-phenanthroline have no effect
Pseudomonas sp.
NaCN
1 mM, 12% inhibition
Pseudomonas sp.
NADH
10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM
Pseudomonas sp.
ZnCl2
1 mM, 46% inhibition
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
CoA
-
Pseudomonas sp.
0.15
-
3-oxoadipyl-CoA
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, SDS-PAGE
Pseudomonas sp.
162000
-
gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.1
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + 3-oxoadipyl-CoA
-
646002
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
-
CoA + 3-oxoadipyl-CoA
-
646002
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
-
CoA + oxoadipyl-CoA
-
646002
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
?
CoA + oxoadipyl-CoA
-
646002
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 42000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.8
-
3-oxoadipyl-CoA
-
Pseudomonas sp.
7.8
-
CoA
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, 90% inhibition
Pseudomonas sp.
additional information
1 mM EDTA or 1,10-phenanthroline have no effect
Pseudomonas sp.
NaCN
1 mM, 12% inhibition
Pseudomonas sp.
NADH
10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM
Pseudomonas sp.
ZnCl2
1 mM, 46% inhibition
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
CoA
-
Pseudomonas sp.
0.15
-
3-oxoadipyl-CoA
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, SDS-PAGE
Pseudomonas sp.
162000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.1
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + 3-oxoadipyl-CoA
-
646002
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
-
CoA + 3-oxoadipyl-CoA
-
646002
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
-
CoA + oxoadipyl-CoA
-
646002
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
?
CoA + oxoadipyl-CoA
-
646002
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 42000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.8
-
3-oxoadipyl-CoA
-
Pseudomonas sp.
7.8
-
CoA
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Pseudomonas sp.
Other publictions for EC 2.3.1.174
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704325
Marin
Modified 3-oxoadipate pathway ...
Pseudomonas reinekei
J. Bacteriol.
192
1543-1552
2010
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
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1
-
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1
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-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646001
Gobel
Degradation of aromatics and c ...
Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
184
216-223
2002
-
-
1
-
-
-
-
-
-
-
1
2
-
24
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
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-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646002
Kaschabek
Degradation of aromatics and c ...
Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
184
207-215
2002
-
-
-
-
-
-
5
2
-
-
2
-
-
22
-
-
1
-
-
-
1
-
4
1
-
-
-
2
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
5
-
2
-
-
2
-
-
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-
1
-
-
1
-
4
1
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-
2
1
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