Application | Comment | Organism |
---|---|---|
agriculture | Ss5MaT1 cDNA may be an important tool for controlling flower colors by metabolic engineering of anthocyanin biosynthesis | Salvia splendens |
Cloned (Comment) | Organism |
---|---|
from petals, expression in Escherichia coli JM109, sequencing | Salvia splendens |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Salvia splendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | 1 mM, 48% inhibition | Salvia splendens | |
caffeic acid | 10 mM, complete inhibition | Salvia splendens | |
Cu2+ | 0.1 mM, complete inhibition | Salvia splendens | |
diethyldicarbonate | 1 mM, 51% inhibition, 20 min, 20°C | Salvia splendens | |
Hg2+ | 0.1 mM, complete inhibition | Salvia splendens | |
additional information | not inhibited by Ca2+, Cd2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Sn2+, Zn2+, all at 0.1 mM, 1 mM dithiothreitol, 1 mM EDTA, 1% Tween 20, 1% Triton X-100, 0.1% CHAPS, 0.1 mM pelargonidin 3,5-diglucoside, 10 mM glucose | Salvia splendens | |
N-ethylmaleimide | 5 mM, 53% inhibition, 20 min, 20°C | Salvia splendens | |
p-coumaric acid | competitive inhibitor with respect to shisonin, 10 mM, complete inhibition | Salvia splendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0141 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, native Ss5MaT1 | Salvia splendens | |
0.0219 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, recombinant Ss5MaT1 | Salvia splendens | |
0.0355 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, recombinant Ss5MaT1 | Salvia splendens | |
0.0402 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, native Ss5MaT1 | Salvia splendens | |
0.057 | - |
malonyl-CoA | pH 7, 30°C, bisdemalonylsalvianin as cosubstrate | Salvia splendens | |
0.101 | - |
pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | pH 7, 30°C, malonyl-CoA as cosubstrate | Salvia splendens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | presumably located in | Salvia splendens | 5829 | - |
soluble | recombinant Ss5MaT1 | Salvia splendens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
gel filtration | Salvia splendens |
50000 | - |
1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation | Salvia splendens |
50723 | - |
1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation | Salvia splendens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salvia splendens | Ss5MaT1 expression is temporally regulated during the color development of the flower, Ss5MaT1 plays a role during the coloration of the flowers, salvianin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia splendens | Q8W1W9 | - |
- |
Oxidation Stability | Organism |
---|---|
extremely unstable to oxidation | Salvia splendens |
Purification (Comment) | Organism |
---|---|
native Ss5MaT1 from flowers: 685fold, recombinant Ss5MaT1 | Salvia splendens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside = CoA + 4'''-demalonylsalvianin | specific for the penultimate step in salvianin biosynthesis, the enzyme also catalyses the malonylation of shisonin to malonylshisonin [cyanidin 3-O-(6''-O-p-coumaryl-beta-D-glucoside)-5-(6'''-O-malonyl-beta-D-glucoside)], the compounds 4'''-demalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside and delphinidin 3,5-diglucoside cannot act as substrates | Salvia splendens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bract | Ss5MaT1 mRNA is present at a lower level than in petals and sepals | Salvia splendens | - |
flower | recently opened flowers, less than 2 cm in length, Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals | Salvia splendens | - |
petal | Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals | Salvia splendens | - |
sepal | Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals | Salvia splendens | - |
stem | red stems, Ss5MaT1 mRNA is present at a lower level than in petals and sepals | Salvia splendens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.17 | - |
pH 7, 30°C | Salvia splendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + shisonin | 2% of the activity with malonyl-CoA as acyl donor | Salvia splendens | CoA + acetylshisonin | - |
? | |
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | Ss5MaT1 catalyzes the regiospecific transfer of the malonyl group from malonyl-CoA to the 6'''-hydroxyl group of the 5-glucosyl moiety of anthocyanins, e.g. bisdemalonylsalvianin, the presence of an aromatic acyl group at the 3-glucosidic moiety of anthocyanin is important for substrate recognition, strong acyl donor preference for malonyl-CoA | Salvia splendens | CoA + 4'''-demalonylsalvianin | - |
? | |
malonyl-CoA + shisonin | Ss5MaT1 catalyzes the regiospecific transfer of the malonyl group from malonyl-CoA to the 6'''-hydroxyl group of the 5-glucosyl moiety of shisonin, enzyme catalyzes exclusively the monomalonylation of shisonin, strong acyl donor preference for malonyl-CoA | Salvia splendens | CoA + 6'''-malonylshisonin | - |
? | |
methylmalonyl-CoA + shisonin | 3% of the activity with malonyl-CoA as acyl donor | Salvia splendens | CoA + methylmalonylshisonin | - |
? | |
additional information | Ss5MaT1 expression is temporally regulated during the color development of the flower, Ss5MaT1 plays a role during the coloration of the flowers, salvianin biosynthesis | Salvia splendens | ? | - |
? | |
additional information | not: 4'''-monodemalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside, delphinidin 3,5-diglucoside, caffeoyl-CoA, p-coumaroyl-CoA, the second malonylation of 4'''-demalonylsalvianin to salvianin is catalyzed by a distinct malonyltransferase | Salvia splendens | ? | - |
? | |
succinyl-CoA + shisonin | 49% of the activity with malonyl-CoA as acyl donor | Salvia splendens | CoA + succinylshisonin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation | Salvia splendens |
Synonyms | Comment | Organism |
---|---|---|
5-O-glucoside-6'''-O-malonyltransferase | - |
Salvia splendens |
anthocyanidin 5-glucoside malonyltransferase | - |
Salvia splendens |
anthocyanidin 5-glycoside malonyltransferase | - |
Salvia splendens |
anthocyanin 5-glycoside malonyltransferase | - |
Salvia splendens |
malonyl-CoA:anthocyanin | - |
Salvia splendens |
malonyl-CoA:anthocyanin 5-O-glucoside-6"'-O-malonyltransferase | - |
Salvia splendens |
malonyltransferase, anthocyanidin 5-glycoside | - |
Salvia splendens |
More | member of the versatile plant acyltransferase family | Salvia splendens |
Ss5MaT1 | - |
Salvia splendens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salvia splendens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7, 20 min, stable below 30°C | Salvia splendens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, native Ss5MaT1 | Salvia splendens | |
7.8 | - |
pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | pH 7, 30°C, malonyl-CoA as cosubstrate | Salvia splendens | |
8.7 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, recombinant Ss5MaT1 | Salvia splendens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Salvia splendens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | active in the range | Salvia splendens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
20°C, 20 h, stable | Salvia splendens |