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Literature summary for 2.3.1.169 extracted from

  • Zhu, X.; Li, T.; Gu, X.; Zhang, S.; Liu, Y.; Wang, Y.; Tan, X.
    Structural and functional investigation into acetyl-coenzyme A synthase and methyltransferase from human pathogen Clostridium difficile (2013), Metallomics, 5, 551-558.
    View publication on PubMed
Search for more literature for 2.3.1.169

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridioides difficile

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, complete inhibition. 1,10-Phenanthroline additionally has inhibitory effects on growth of Peptoclostridium difficile Clostridioides difficile
2,2-dipyridyl 1 mM, complete inhibition. 2,2-Dipyridyl additionally has inhibitory effects on growth of Peptoclostridium difficile Clostridioides difficile

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center Clostridioides difficile
Nickel protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-
Clostridioides difficile 630
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
-
 Clostridioides difficile CH3-CO-S-CoA + corrinoid/iron-sulfur protein
-
 ?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
-
 Clostridioides difficile 630 CH3-CO-S-CoA + corrinoid/iron-sulfur protein
-
 ?

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid] [Fe4S4] center. Preparation shows a broad absorption at the 420 nm region Clostridioides difficile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.66
-
 HS-CoA at 1 atm CO, pH not specified in the publication, temperature not specified in the publication Clostridioides difficile