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Literature summary for 2.3.1.169 extracted from
- Methods for analysis of acetyl-CoA synthase applications to bacterial and archaeal systems (2011), Methods Enzymol., 494, 189-217.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Carboxydothermus hydrogenoformans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co3+ | part of the methylcorrinoid protein | Carboxydothermus hydrogenoformans |  |
| additional information | Ti3+ NTAis utilized in the CO exchange assay | Carboxydothermus hydrogenoformans | |
| Ni2+ | required as reductant of the methylcorrinoid protein | Carboxydothermus hydrogenoformans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + corrinoid protein | Carboxydothermus hydrogenoformans | - |
CoA + CO + methylcorrinoid protein | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carboxydothermus hydrogenoformans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + corrinoid protein | - |
Carboxydothermus hydrogenoformans | CoA + CO + methylcorrinoid protein | - |
r | |
| additional information | in assays of bacterial ACS, methylated corrinoid iron-sulfur protein is generally used as the source of methyl groups for acetyl-CoA synthesis | Carboxydothermus hydrogenoformans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACSCh | - |
Carboxydothermus hydrogenoformans |
| CODH/ACS | - |
Carboxydothermus hydrogenoformans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
acetyl-CoA synthesis and CO exchange assay at | Carboxydothermus hydrogenoformans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
CO exchange assay at | Carboxydothermus hydrogenoformans |
| 7.2 | - |
acetyl-CoA synthesis assay at | Carboxydothermus hydrogenoformans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CODH/ACS is the only protein required to catalyze exchange of the carbonyl group of acetyl-CoA with free CO. CODH/ACS acts on both the acetyl C-C and C-S bonds in acetyl-CoA, and it identified CODH/ACS as the condensing enzyme that catalyzes the final steps in acetyl-CoA synthesis in acetogenic bacteria | Carboxydothermus hydrogenoformans |
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Release 2023.1 (January 2023)
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