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Literature summary for 2.3.1.169 extracted from
- Catalysis of acetyl-CoA cleavage and tetrahydrosarcinapterin methylation by a carbon monoxide dehydrogenase-corrinoid enzyme complex (1991), J. Biol. Chem., 266, 22227-22233.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | corrinoid/iron-sulfur protein required | Methanosarcina barkeri |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19700 | - |
alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
| 51400 | - |
alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
| 53000 | - |
alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
| 63200 | - |
alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
| 84500 | - |
alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
| 1600000 | - |
gel filtration | Methanosarcina barkeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O | the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the cleavage of acetyl-CoA, tetrahydrosarcinapterin functions as the methyl group acceptor, the major products of reaction are methyltetrahydrosarcinapterin and CO2, free CoA is identified as an additional product | Methanosarcina barkeri | CH3-tetrahydrosarcinapterin + CO2 + H+ + electron | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| polymer | alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE | Methanosarcina barkeri |
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Release 2023.1 (January 2023)
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