BRENDA - Enzyme Database show
show all sequences of 2.3.1.168

Catalytic and structural properties of the dihydrolipoyl transacylase component of bovine branched-chain alpha-keto acid dehydrogenase

Chuang, D.T.; Hu, C.C.; Ku, L.S.; Niu, W.L.; Myers, D.E.; Cox, R.P.; J. Biol. Chem. 259, 9277-9284 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
strong inhibition
Bos taurus
CoA
product inhibition, competitive to acyl-CoA, noncompetitive to dihydrolipoamide
Bos taurus
Mg2+
-
Bos taurus
additional information
limited proteolysis by trypsin results in the complete loss of the overall activity of the enzyme complex, but does not affect dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; NEM and thiamine diphosphate have no effect
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bos taurus
0.05
-
isovaleryl-CoA
pH 7.4, 37°C
Bos taurus
0.1
-
isobutyryl-CoA
pH 7.4, 37°C
Bos taurus
0.11
-
acetyl-CoA
pH 7.4, 37°C
Bos taurus
2
-
6,8-bis(sulfanyl)octanamide
pH 7.4, 37°C
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52600
-
x * 52600, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
Bos taurus
-
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
Bos taurus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Reaction
Reaction
Commentary
Organism
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
random bi bi mechanism
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Bos taurus
-
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.38
-
partially purified E2 enzyme
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylpropanoyl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-2-methylpropanoyldihydrolipoamide
identification by mass spectrometry
644839
Bos taurus
r
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
644839
Bos taurus
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
644839
Bos taurus
r
acetyl-CoA + dihydrolipoamide
low activity
644839
Bos taurus
CoA + S-acetyldihydrolipoamide
-
644839
Bos taurus
r
isobutyryl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-isobutyryldihydrolipoamide
-
644839
Bos taurus
r
isovaleryl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-isovaleryldihydrolipoamide
-
644839
Bos taurus
r
Subunits
Subunits
Commentary
Organism
?
x * 52600, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
strong inhibition
Bos taurus
CoA
product inhibition, competitive to acyl-CoA, noncompetitive to dihydrolipoamide
Bos taurus
Mg2+
-
Bos taurus
additional information
limited proteolysis by trypsin results in the complete loss of the overall activity of the enzyme complex, but does not affect dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; NEM and thiamine diphosphate have no effect
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bos taurus
0.05
-
isovaleryl-CoA
pH 7.4, 37°C
Bos taurus
0.1
-
isobutyryl-CoA
pH 7.4, 37°C
Bos taurus
0.11
-
acetyl-CoA
pH 7.4, 37°C
Bos taurus
2
-
6,8-bis(sulfanyl)octanamide
pH 7.4, 37°C
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52600
-
x * 52600, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
Bos taurus
-
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
Bos taurus
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Bos taurus
-
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.38
-
partially purified E2 enzyme
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylpropanoyl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-2-methylpropanoyldihydrolipoamide
identification by mass spectrometry
644839
Bos taurus
r
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
644839
Bos taurus
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
644839
Bos taurus
r
acetyl-CoA + dihydrolipoamide
low activity
644839
Bos taurus
CoA + S-acetyldihydrolipoamide
-
644839
Bos taurus
r
isobutyryl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-isobutyryldihydrolipoamide
-
644839
Bos taurus
r
isovaleryl-CoA + dihydrolipoamide
-
644839
Bos taurus
CoA + S-isovaleryldihydrolipoamide
-
644839
Bos taurus
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 52600, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bos taurus
Other publictions for EC 2.3.1.168
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704473
Brautigam
Subunit and catalytic componen ...
Homo sapiens
J. Biol. Chem.
284
13086-13098
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702242
Klyuyeva
Allosteric coupling in pyruvat ...
Rattus norvegicus
Biochemistry
47
8358-8366
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644848
Hakozaki
Characterization of rainbow tr ...
Gallus gallus, Oncorhynchus mykiss
Comp. Biochem. Physiol. B Biochem. Mol. Biol.
132
433-442
2002
-
-
1
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
1
1
1
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
1
1
1
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644846
Perham
Swinging arms and swinging dom ...
Acidithiobacillus ferrooxidans, Azotobacter vinelandii, Cupriavidus necator, Enterococcus faecalis, Escherichia coli, Geobacillus stearothermophilus, Haemophilus influenzae, Homo sapiens, Neisseria meningitidis, no activity in archaebacteria, no activity in Desulfovibrio africanus, Saccharomyces cerevisiae, Zymomonas mobilis
Annu. Rev. Biochem.
69
961-1004
2000
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
-
-
-
-
22
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
22
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644847
Chuang
Production of recombinant mamm ...
Bos taurus
Methods Enzymol.
324
192-200
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644845
Ansari
Epitope mapping of the branche ...
Bos taurus, Homo sapiens
J. Immunol.
153
4754-4765
1994
-
-
1
-
-
-
2
-
2
-
-
1
-
2
-
-
2
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
2
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644849
Wynn
Invitro reconstitution of the ...
Bos taurus
Biochemistry
33
8962-8968
1994
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644843
Lau
Structure of the gene encoding ...
Homo sapiens
J. Biol. Chem.
267
24090-24096
1992
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644844
Lau
The complete cDNA sequence for ...
Homo sapiens
Biochim. Biophys. Acta
1132
319-321
1992
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644842
Chuang
Maple syrup urine disease: dom ...
Bos taurus, Homo sapiens
Mol. Biol. Med.
8
49-63
1991
-
-
2
-
3
-
-
-
-
-
2
2
-
2
-
-
1
1
-
2
1
-
4
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
2
2
-
-
-
1
-
2
1
-
4
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644841
Hu
Subunit structure of the dihyd ...
Bos taurus
J. Biol. Chem.
261
343-349
1986
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644840
Chuang
Subunit structure of the dihyd ...
Bos taurus
J. Biol. Chem.
260
13779-13786
1985
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
644839
Chuang
Catalytic and structural prope ...
Bos taurus
J. Biol. Chem.
259
9277-9284
1984
-
-
-
-
-
-
4
5
-
-
1
1
-
1
-
-
1
1
-
2
1
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
1
1
-
-
-
1
-
2
1
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-