Any feedback?
Literature summary for 2.3.1.168 extracted from
- Catalytic and structural properties of the dihydrolipoyl transacylase component of bovine branched-chain alpha-keto acid dehydrogenase (1984), J. Biol. Chem., 259, 9277-9284.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | strong inhibition | Bos taurus |  |
| CoA | product inhibition, competitive to acyl-CoA, noncompetitive to dihydrolipoamide | Bos taurus | |
| Mg2+ | - |
Bos taurus | |
| additional information | limited proteolysis by trypsin results in the complete loss of the overall activity of the enzyme complex, but does not affect dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; NEM and thiamine diphosphate have no effect | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bos taurus | |
| 0.05 | - |
isovaleryl-CoA | pH 7.4, 37°C | Bos taurus | |
| 0.1 | - |
isobutyryl-CoA | pH 7.4, 37°C | Bos taurus | |
| 0.11 | - |
acetyl-CoA | pH 7.4, 37°C | Bos taurus | |
| 2 | - |
6,8-bis(sulfanyl)octanamide | pH 7.4, 37°C | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52600 | - |
x * 52600, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | Bos taurus | - |
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | random bi bi mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.38 | - |
partially purified E2 enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-2-methylpropanoyldihydrolipoamide | identification by mass spectrometry | r | |
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
Bos taurus | CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
r | |
| acetyl-CoA + dihydrolipoamide | low activity | Bos taurus | CoA + S-acetyldihydrolipoamide | - |
r | |
| isobutyryl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-isobutyryldihydrolipoamide | - |
r | |
| isovaleryl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-isovaleryldihydrolipoamide | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52600, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
