Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | strong inhibition | Bos taurus | |
CoA | product inhibition, competitive to acyl-CoA, noncompetitive to dihydrolipoamide | Bos taurus | |
Mg2+ | - |
Bos taurus | |
additional information | limited proteolysis by trypsin results in the complete loss of the overall activity of the enzyme complex, but does not affect dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; NEM and thiamine diphosphate have no effect | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bos taurus | |
0.05 | - |
isovaleryl-CoA | pH 7.4, 37°C | Bos taurus | |
0.1 | - |
isobutyryl-CoA | pH 7.4, 37°C | Bos taurus | |
0.11 | - |
acetyl-CoA | pH 7.4, 37°C | Bos taurus | |
2 | - |
6,8-bis(sulfanyl)octanamide | pH 7.4, 37°C | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52600 | - |
x * 52600, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | Bos taurus | - |
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | random bi bi mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.38 | - |
partially purified E2 enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylpropanoyl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-2-methylpropanoyldihydrolipoamide | identification by mass spectrometry | r | |
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
Bos taurus | CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
r | |
acetyl-CoA + dihydrolipoamide | low activity | Bos taurus | CoA + S-acetyldihydrolipoamide | - |
r | |
isobutyryl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-isobutyryldihydrolipoamide | - |
r | |
isovaleryl-CoA + dihydrolipoamide | - |
Bos taurus | CoA + S-isovaleryldihydrolipoamide | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 52600, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |