General Stability | Organism |
---|---|
the recombinant DBAT is immobilized by cross-linked enzyme aggregates. To further optimize the enzyme recovery, single-factor experiment and response surface methodology is applied. 60% ammonium sulfate as precipitant, 0.05% glutaraldehyde as fixing agent, pH 7.0, 2 h as cross-linking time, 30°C as cross-linking temperature are confirmed to be the optimum conditions. 62% for ammonium sulfate saturation, 0.15% for glutaraldehyde, and pH 6.75 are the optimum conditions with averagely 73.9% activity recovery in 3 replications | Taxus mairei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus mairei | H9BIH8 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DBAT | - |
Taxus mairei |
taxoid 10beta-O-acetyltransferase | - |
Taxus mairei |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme to produce baccatin III, a key precursor in paclitaxel synthesis | Taxus mairei |