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Literature summary for 2.3.1.167 extracted from

  • You, L.; Wei, T.; Zheng, Q.; Lin, J.; Guo, L.; Jiang, B.; Huang, J.
    Activity essential residue analysis of taxoid 10beta-O-acetyl transferase for enzymatic synthesis of baccatin (2018), Appl. Biochem. Biotechnol., 186, 949-959 .
    View publication on PubMed
Search for more literature for 2.3.1.167

Application

Application Comment Organism
pharmacology key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III Taxus mairei
pharmacology paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production Taxus mairei
synthesis key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III Taxus mairei
synthesis paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production Taxus mairei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Taxus mairei

Protein Variants

Protein Variants Comment Organism
D166A decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei
D34E decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei
D372E decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei
G376A decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei
H162A mutation results in complete loss of enzymatic activity, suggesting that the residue histidine at 162 is essential to DBAT activity Taxus mairei
R363A decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei
S31A decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA Taxus mairei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.073
-
 acetyl-CoA wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei
0.075
-
 10-deacetylbaccatin III wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei
0.134
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.148
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.231
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.29
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.762
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
0.925
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
1.094
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
1.51
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
1.67
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
2.28
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
3.21
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III Taxus mairei
-
 CoA + baccatin III
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus mairei H9BIH8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus mairei CoA + baccatin III
-
 ?
N-acetyl-D-glucosamine + 10-deacetylbaccatin III the yield when using N-acetyl-D-glucosamine as acetyl donor in open-whole-cell catalytic system is 2.13fold of that when using acetyl-CoA. In the in vivo system, the yield increased 24.17%, which may indicate its cooperation with acetyl-CoA Taxus mairei D-glucosamine + baccatin III
-
 ?
vinyl acetate + 10-deacetylbaccatin III 42% of the activity as compared to N-acetyl-D-glucosamine Taxus mairei vinyl alcohol + baccatin III
-
 ?

Synonyms

Synonyms Comment Organism
DBAT
-
 Taxus mairei
taxoid 10beta-O-acetyl transferase
-
 Taxus mairei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.59
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
5.28
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
12.73
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
14.69
-
 10-deacetylbaccatin III wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei
16.12
-
 acetyl-CoA wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei
18.79
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
20.54
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
21.74
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
26.65
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
27.03
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
27.61
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
33.12
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
10
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
20
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
20
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
30
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
40
-
 acetyl-CoA mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
70
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
120
-
 10-deacetylbaccatin III mutant enzyme, 30°C, pH not specified in the publication Taxus mairei
200
-
 10-deacetylbaccatin III wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei
220
-
 acetyl-CoA wild-type enzyme, 30°C, pH not specified in the publication Taxus mairei