BRENDA - Enzyme Database show
show all sequences of 2.3.1.167

The relationship between TXS, DBAT, BAPT and DBTNBT gene expression and taxane production during the development of Taxus baccata plantlets

Onrubia, M.; Moyano, E.; Bonfill, M.; Palazon, J.; Goossens, A.; Cusido, R.M.; Plant Sci. 181, 282-287 (2011)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
Taxus baccata
-
CoA + baccatin III
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus baccata
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
aerial part
-
Taxus baccata
-
additional information
higher content of 10-deacetylbaccatin III in the aerial part of the plantlets than in the roots
Taxus baccata
-
root
-
Taxus baccata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
720764
Taxus baccata
CoA + baccatin III
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
Taxus baccata
-
CoA + baccatin III
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
aerial part
-
Taxus baccata
-
additional information
higher content of 10-deacetylbaccatin III in the aerial part of the plantlets than in the roots
Taxus baccata
-
root
-
Taxus baccata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
720764
Taxus baccata
CoA + baccatin III
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the relationship between taxane patterns and the expression of genes encoding the enzymes taxadiene synthase, 10-deacetylbaccatin III-10beta-O-acetyltransferase, baccatin III 13-O-(3-amino-3-phenylpropanoyl) transferase and 3'-N-debenzoyl-2'-deoxytaxol-N-benzoyltransferase, involved in early and late steps of the taxane pathway, overview. The most abundant taxane in the aerial parts is 10-deacetylbaccatin III, which increases as the plantlets grow, indicating a low conversion to baccatin III and taxol. In contrast, the levels of 10-deacetylbaccatin III in the roots remain lower than those of taxol. But in the aerial parts the expression of DBAT, which codes for the enzyme that converts 10-deacetylbaccatin III into baccatin III, does not increase with the age of plantlets, unlike that of the other three enzymes, suggesting that this gene controls a rate-limiting step in the taxane biosynthetic pathway
Taxus baccata
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the relationship between taxane patterns and the expression of genes encoding the enzymes taxadiene synthase, 10-deacetylbaccatin III-10beta-O-acetyltransferase, baccatin III 13-O-(3-amino-3-phenylpropanoyl) transferase and 3'-N-debenzoyl-2'-deoxytaxol-N-benzoyltransferase, involved in early and late steps of the taxane pathway, overview. The most abundant taxane in the aerial parts is 10-deacetylbaccatin III, which increases as the plantlets grow, indicating a low conversion to baccatin III and taxol. In contrast, the levels of 10-deacetylbaccatin III in the roots remain lower than those of taxol. But in the aerial parts the expression of DBAT, which codes for the enzyme that converts 10-deacetylbaccatin III into baccatin III, does not increase with the age of plantlets, unlike that of the other three enzymes, suggesting that this gene controls a rate-limiting step in the taxane biosynthetic pathway
Taxus baccata
Other publictions for EC 2.3.1.167
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Han
Bioproduction of baccatin III, ...
Taxus wallichiana var. chinensis, Taxus wallichiana var. chinensis var. mairei
J. Sci. Food Agric.
94
2376-2383
2014
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720764
Onrubia
The relationship between TXS, ...
Taxus baccata
Plant Sci.
181
282-287
2011
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1
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1
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719235
Zhang
Effects of different elicitors ...
Taxus cuspidata, Taxus cuspidata T5
Cell Biol. Int.
18
e00009
2010
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1
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1
1
1
1
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703336
Zhang
An endophytic taxol-producing ...
Cladosporium cladosporioides
Curr. Microbiol.
59
227-232
2009
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7
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703751
Zhang
An endophytic taxol-producing ...
Aspergillus candidus
FEMS Microbiol. Lett.
293
155-159
2009
-
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-
-
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4
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685463
Hao
Phylogenetic relationships of ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus floridana, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana
Biol. Pharm. Bull.
31
260-265
2008
-
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11
-
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13
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11
-
22
-
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11
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11
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11
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13
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11
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11
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11
-
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686959
Hao
Molecular evolution of paclita ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus cuspidata, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana, Taxus x hunnewelliana, Taxus x media
Genetica
135
123-135
2008
-
-
12
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15
-
15
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15
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15
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15
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15
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704167
Ondari
The taxol pathway 10-O-acetylt ...
Taxus cuspidata
J. Am. Chem. Soc.
130
17187-17194
2008
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1
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1
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3
-
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1
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-
3
3
706930
Katkovcinova
Expression of dbat and dbtnbt ...
Taxus baccata
Z. Naturforsch. C
63
721-730
2008
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1
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1
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1
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-
672726
Loncaric
Expression of an acetyl-CoA sy ...
Taxus sp.
Biotechnol. J.
2
266-274
2007
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2
1
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3
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5
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5
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1
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1
1
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5
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688912
Guo
Molecular cloning and heterolo ...
Taxus x media
Mol. Biol. Rep.
34
89-95
2007
3
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1
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1
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3
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1
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1
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672704
DeJong
Genetic engineering of taxol b ...
Taxus brevifolia
Biotechnol. Bioeng.
93
212-224
2006
-
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1
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1
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673024
Loncaric
Profiling a taxol pathway 10be ...
Taxus sp.
Chem. Biol.
13
309-317
2006
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1
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660432
Fang
Expression cloned cDNA for 10- ...
Taxus baccata
Protein Expr. Purif.
35
17-24
2004
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1
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2
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1
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440288
Walker
Taxol biosynthetic genes ...
Taxus cuspidata
Phytochemistry
58
1-7
2001
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486453
Walker.K.; Croteau
Molecular cloning of a 10-deac ...
Taxus cuspidata
Proc. Natl. Acad. Sci. USA
97
583-587
2000
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1
1
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2
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2
1
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6
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1
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1
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1
1
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486455
Pennington
-
Acetyl CoA: 10-deacetylbaccati ...
Taxus cuspidata
Phytochemistry
49
2261-2266
1998
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2
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