BRENDA - Enzyme Database show
show all sequences of 2.3.1.167

Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media

Guo, B.; Kai, G.; Gong, Y.; Jin, H.; Wang, Y.; Miao, Z.; Sun, X.; Tang, K.; Mol. Biol. Rep. 34, 89-95 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ammonium ceric(IV) sulfate
-
Taxus x media
methyl jasmonate
-
Taxus x media
Silver nitrate
-
Taxus x media
Cloned(Commentary)
Commentary
Organism
the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells
Taxus x media
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
tag-protein
Taxus x media
48980
-
predicted molecular mass
Taxus x media
65000
-
determined by SDS-PAGE, TmDBAT plus fused tag
Taxus x media
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
Taxus x media
-
CoA + baccatin III
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus x media
Q6SQJ5
-
-
Purification (Commentary)
Commentary
Organism
by affinity chromatography in Ni-nitrilotriacetic acid columns, the tag is removed by digestion with enterokinase
Taxus x media
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Taxus x media
-
stem
-
Taxus x media
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
688912
Taxus x media
CoA + baccatin III
-
-
-
?
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Taxus x media
predicted pI-value
-
6.05
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ammonium ceric(IV) sulfate
-
Taxus x media
methyl jasmonate
-
Taxus x media
Silver nitrate
-
Taxus x media
Cloned(Commentary) (protein specific)
Commentary
Organism
the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells
Taxus x media
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
tag-protein
Taxus x media
48980
-
predicted molecular mass
Taxus x media
65000
-
determined by SDS-PAGE, TmDBAT plus fused tag
Taxus x media
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
Taxus x media
-
CoA + baccatin III
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
by affinity chromatography in Ni-nitrilotriacetic acid columns, the tag is removed by digestion with enterokinase
Taxus x media
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Taxus x media
-
stem
-
Taxus x media
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
688912
Taxus x media
CoA + baccatin III
-
-
-
?
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Taxus x media
predicted pI-value
-
6.05
Other publictions for EC 2.3.1.167
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736700
Han
Bioproduction of baccatin III, ...
Taxus wallichiana var. chinensis, Taxus wallichiana var. chinensis var. mairei
J. Sci. Food Agric.
94
2376-2383
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720764
Onrubia
The relationship between TXS, ...
Taxus baccata
Plant Sci.
181
282-287
2011
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719235
Zhang
Effects of different elicitors ...
Taxus cuspidata, Taxus cuspidata T5
Cell Biol. Int.
18
e00009
2010
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
703336
Zhang
An endophytic taxol-producing ...
Cladosporium cladosporioides
Curr. Microbiol.
59
227-232
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703751
Zhang
An endophytic taxol-producing ...
Aspergillus candidus
FEMS Microbiol. Lett.
293
155-159
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685463
Hao
Phylogenetic relationships of ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus floridana, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana
Biol. Pharm. Bull.
31
260-265
2008
-
-
11
-
-
-
-
-
13
-
-
11
-
22
-
-
-
-
-
11
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
13
-
-
11
-
-
-
-
-
11
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686959
Hao
Molecular evolution of paclita ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus cuspidata, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana, Taxus x hunnewelliana, Taxus x media
Genetica
135
123-135
2008
-
-
12
-
-
-
-
-
-
-
-
15
-
15
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704167
Ondari
The taxol pathway 10-O-acetylt ...
Taxus cuspidata
J. Am. Chem. Soc.
130
17187-17194
2008
-
-
-
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
3
3
706930
Katkovcinova
Expression of dbat and dbtnbt ...
Taxus baccata
Z. Naturforsch. C
63
721-730
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
672726
Loncaric
Expression of an acetyl-CoA sy ...
Taxus sp.
Biotechnol. J.
2
266-274
2007
-
2
1
-
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688912
Guo
Molecular cloning and heterolo ...
Taxus x media
Mol. Biol. Rep.
34
89-95
2007
3
-
1
-
-
-
-
-
-
-
3
1
-
3
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
672704
DeJong
Genetic engineering of taxol b ...
Taxus brevifolia
Biotechnol. Bioeng.
93
212-224
2006
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673024
Loncaric
Profiling a taxol pathway 10be ...
Taxus sp.
Chem. Biol.
13
309-317
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660432
Fang
Expression cloned cDNA for 10- ...
Taxus baccata
Protein Expr. Purif.
35
17-24
2004
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
3
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
3
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
440288
Walker
Taxol biosynthetic genes ...
Taxus cuspidata
Phytochemistry
58
1-7
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486453
Walker.K.; Croteau
Molecular cloning of a 10-deac ...
Taxus cuspidata
Proc. Natl. Acad. Sci. USA
97
583-587
2000
-
1
1
-
-
-
-
2
-
-
2
1
-
6
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
486455
Pennington
-
Acetyl CoA: 10-deacetylbaccati ...
Taxus cuspidata
Phytochemistry
49
2261-2266
1998
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-