BRENDA - Enzyme Database show
show all sequences of 2.3.1.167

Expression cloned cDNA for 10-deacetylbaccatin III-10-O-acetyltransferase in Escherichia coli: a comparative study of three fusion systems

Fang, J.; Ewald, D.; Protein Expr. Purif. 35, 17-24 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag
Taxus baccata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
acetyl-CoA
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
0.011
-
10-deacetylbaccatin III
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus baccata
-
-
-
Purification (Commentary)
Commentary
Organism
-
Taxus baccata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.1
-
recombinant enzyme with biotinylated peptide tag
Taxus baccata
16.1
-
recombinant glutathione S-transferase-fusion protein
Taxus baccata
24.4
-
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
660432
Taxus baccata
CoA + baccatin III
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Taxus baccata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
recombinant enzymes
Taxus baccata
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
half-maximal aactivity of recombinant enzyme at pH 6.0 and 8.0
Taxus baccata
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag
Taxus baccata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
acetyl-CoA
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
0.011
-
10-deacetylbaccatin III
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
Purification (Commentary) (protein specific)
Commentary
Organism
-
Taxus baccata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.1
-
recombinant enzyme with biotinylated peptide tag
Taxus baccata
16.1
-
recombinant glutathione S-transferase-fusion protein
Taxus baccata
24.4
-
recombinant enzyme with a hexahistidine fusion tag
Taxus baccata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 10-deacetylbaccatin III
-
660432
Taxus baccata
CoA + baccatin III
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Taxus baccata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
recombinant enzymes
Taxus baccata
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
half-maximal aactivity of recombinant enzyme at pH 6.0 and 8.0
Taxus baccata
Other publictions for EC 2.3.1.167
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736700
Han
Bioproduction of baccatin III, ...
Taxus wallichiana var. chinensis, Taxus wallichiana var. chinensis var. mairei
J. Sci. Food Agric.
94
2376-2383
2014
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1
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2
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720764
Onrubia
The relationship between TXS, ...
Taxus baccata
Plant Sci.
181
282-287
2011
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1
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3
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1
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1
1
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-
719235
Zhang
Effects of different elicitors ...
Taxus cuspidata, Taxus cuspidata T5
Cell Biol. Int.
18
e00009
2010
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1
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4
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1
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1
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1
1
1
1
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-
703336
Zhang
An endophytic taxol-producing ...
Cladosporium cladosporioides
Curr. Microbiol.
59
227-232
2009
-
-
-
-
-
-
-
-
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6
-
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-
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-
703751
Zhang
An endophytic taxol-producing ...
Aspergillus candidus
FEMS Microbiol. Lett.
293
155-159
2009
-
-
-
-
-
-
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4
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-
685463
Hao
Phylogenetic relationships of ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus floridana, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana
Biol. Pharm. Bull.
31
260-265
2008
-
-
11
-
-
-
-
-
13
-
-
11
-
22
-
-
-
-
-
11
-
-
11
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11
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13
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11
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11
-
-
11
-
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-
-
-
-
-
-
-
-
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-
-
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-
686959
Hao
Molecular evolution of paclita ...
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus cuspidata, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana var. chinensis, Taxus wallichiana var. wallichiana, Taxus x hunnewelliana, Taxus x media
Genetica
135
123-135
2008
-
-
12
-
-
-
-
-
-
-
-
15
-
15
-
-
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-
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15
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15
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15
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-
-
-
15
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-
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-
704167
Ondari
The taxol pathway 10-O-acetylt ...
Taxus cuspidata
J. Am. Chem. Soc.
130
17187-17194
2008
-
-
-
-
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1
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1
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3
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1
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3
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1
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1
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1
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1
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1
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3
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1
-
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-
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-
3
3
706930
Katkovcinova
Expression of dbat and dbtnbt ...
Taxus baccata
Z. Naturforsch. C
63
721-730
2008
-
-
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-
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1
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1
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1
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1
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1
-
-
672726
Loncaric
Expression of an acetyl-CoA sy ...
Taxus sp.
Biotechnol. J.
2
266-274
2007
-
2
1
-
-
-
-
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3
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5
-
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-
-
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5
-
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1
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2
1
1
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3
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5
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-
688912
Guo
Molecular cloning and heterolo ...
Taxus x media
Mol. Biol. Rep.
34
89-95
2007
3
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1
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-
-
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3
1
-
3
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1
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2
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1
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1
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1
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3
1
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1
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2
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1
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1
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-
-
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-
672704
DeJong
Genetic engineering of taxol b ...
Taxus brevifolia
Biotechnol. Bioeng.
93
212-224
2006
-
-
-
-
1
-
-
-
-
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1
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1
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1
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-
673024
Loncaric
Profiling a taxol pathway 10be ...
Taxus sp.
Chem. Biol.
13
309-317
2006
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1
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1
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3
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660432
Fang
Expression cloned cDNA for 10- ...
Taxus baccata
Protein Expr. Purif.
35
17-24
2004
-
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1
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2
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4
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1
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3
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1
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1
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1
1
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1
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1
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3
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1
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1
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1
1
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440288
Walker
Taxol biosynthetic genes ...
Taxus cuspidata
Phytochemistry
58
1-7
2001
-
-
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1
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2
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1
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486453
Walker.K.; Croteau
Molecular cloning of a 10-deac ...
Taxus cuspidata
Proc. Natl. Acad. Sci. USA
97
583-587
2000
-
1
1
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-
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2
-
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2
1
-
6
-
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1
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1
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1
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1
1
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1
1
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2
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2
1
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1
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1
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1
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1
1
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486455
Pennington
-
Acetyl CoA: 10-deacetylbaccati ...
Taxus cuspidata
Phytochemistry
49
2261-2266
1998
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2
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1
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