BRENDA - Enzyme Database show
show all sequences of 2.3.1.165

Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum

Spencer, J.B.; Jordan, P.M.; Biochem. J. 288, 839-846 (1992)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
PMSF and benzamidine in disruption buffer extend half-life from 20 min to 6 h, 15% glycerol and proteinase inhibitors further improve stability
Penicillium griseofulvum
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,3-dibromo-propane-2-one
0.02 mM, complete inactivation after 40 min, acetyl-CoA protects
Penicillium griseofulvum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
malonyl-CoA
-
Penicillium griseofulvum
0.01
-
acetyl-CoA
-
Penicillium griseofulvum
0.012
-
NADPH
-
Penicillium griseofulvum
0.065
-
acetoacetyl-CoA
-
Penicillium griseofulvum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
180000
-
alpha4, 4 * 180000, SDS-PAGE
Penicillium griseofulvum
750000
-
gel filtration
Penicillium griseofulvum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Penicillium griseofulvum
-
also known as Penicillium urticae; NRRL 2159A
-
Penicillium griseofulvum NRRL 2159A
-
NRRL 2159A
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, poly(ethylene glycol) 6000, DEAE-Sepharose, hydroxyapatite, Mono Q
Penicillium griseofulvum
Reaction
Reaction
Commentary
Organism
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O
a multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein, it has a similar sequence to vertebrate type I fatty acid synthase, acetoacetyl-CoA can also act as a starter molecule
Penicillium griseofulvum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.245
-
-
Penicillium griseofulvum
Storage Stability
Storage Stability
Organism
-70░C, ammonium sulfate precipitate, loss of 5% activity per month
Penicillium griseofulvum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum
?
-
-
-
?
acetoacetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum NRRL 2159A
?
-
-
-
?
acetyl-CoA + malonyl-CoA
exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH
486447
Penicillium griseofulvum
triacetic lactone
-
486447
Penicillium griseofulvum
?
acetyl-CoA + malonyl-CoA
exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH
486447
Penicillium griseofulvum NRRL 2159A
triacetic lactone
-
486447
Penicillium griseofulvum NRRL 2159A
?
acetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum
6-methylsalicylate + CoA + CO2 + NADP+
-
486447
Penicillium griseofulvum
-
acetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum NRRL 2159A
6-methylsalicylate + CoA + CO2 + NADP+
-
486447
Penicillium griseofulvum NRRL 2159A
-
Subunits
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 180000, SDS-PAGE
Penicillium griseofulvum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Penicillium griseofulvum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Penicillium griseofulvum
General Stability (protein specific)
General Stability
Organism
PMSF and benzamidine in disruption buffer extend half-life from 20 min to 6 h, 15% glycerol and proteinase inhibitors further improve stability
Penicillium griseofulvum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,3-dibromo-propane-2-one
0.02 mM, complete inactivation after 40 min, acetyl-CoA protects
Penicillium griseofulvum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
malonyl-CoA
-
Penicillium griseofulvum
0.01
-
acetyl-CoA
-
Penicillium griseofulvum
0.012
-
NADPH
-
Penicillium griseofulvum
0.065
-
acetoacetyl-CoA
-
Penicillium griseofulvum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
180000
-
alpha4, 4 * 180000, SDS-PAGE
Penicillium griseofulvum
750000
-
gel filtration
Penicillium griseofulvum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, poly(ethylene glycol) 6000, DEAE-Sepharose, hydroxyapatite, Mono Q
Penicillium griseofulvum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.245
-
-
Penicillium griseofulvum
Storage Stability (protein specific)
Storage Stability
Organism
-70░C, ammonium sulfate precipitate, loss of 5% activity per month
Penicillium griseofulvum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum
?
-
-
-
?
acetoacetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum NRRL 2159A
?
-
-
-
?
acetyl-CoA + malonyl-CoA
exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH
486447
Penicillium griseofulvum
triacetic lactone
-
486447
Penicillium griseofulvum
?
acetyl-CoA + malonyl-CoA
exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH
486447
Penicillium griseofulvum NRRL 2159A
triacetic lactone
-
486447
Penicillium griseofulvum NRRL 2159A
?
acetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum
6-methylsalicylate + CoA + CO2 + NADP+
-
486447
Penicillium griseofulvum
-
acetyl-CoA + malonyl-CoA + NADPH
-
486447
Penicillium griseofulvum NRRL 2159A
6-methylsalicylate + CoA + CO2 + NADP+
-
486447
Penicillium griseofulvum NRRL 2159A
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 180000, SDS-PAGE
Penicillium griseofulvum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Penicillium griseofulvum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Penicillium griseofulvum
Other publictions for EC 2.3.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735433
Parascandolo
Insights into 6-methylsalicyli ...
Penicillium griseofulvum
Angew. Chem. Int. Ed. Engl.
55
3463-3467
2016
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1
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1
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1
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3
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1
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1
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-
-
3
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735950
Petersen
Investigation of a 6-MSA synth ...
Aspergillus aculeatus
ChemBioChem
16
2200-2204
2015
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3
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1
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1
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1
1
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735929
Holm
Molecular and chemical charact ...
Aspergillus niger, Aspergillus niger ATCC 1015
Chem. Biol.
21
519-529
2014
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1
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4
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2
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1
1
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719864
Moriguchi
Hidden function of catalytic d ...
Aspergillus terreus
J. Biol. Chem.
285
15637-15643
2010
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1
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2
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1
2
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2
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1
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3
2
1
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1
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1
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2
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1
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1
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3
2
1
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2
1
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2
2
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690561
Panagiotou
Studies on the production of f ...
Penicillium griseofulvum
Appl. Environ. Microbiol.
75
2212-2220
2009
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2
1
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6
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703577
Sanzani
Effect of quercetin and umbell ...
Penicillium expansum
Eur. J. Plant Pathol.
125
223-233
2009
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1
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2
1
1
2
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703824
Bacha
Cloning and characterization o ...
Aspergillus westerdijkiae
Fungal Genet. Biol.
46
742-749
2009
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-
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1
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1
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5
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1
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1
1
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703206
Moriguchi
Domain-domain interactions in ...
Aspergillus terreus, Penicillium griseofulvum
ChemBioChem
9
1207-1212
2008
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1
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5
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1
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3
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2
2
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1
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5
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1
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2
2
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705512
Wattanachaisaereekul
Production of the polyketide 6 ...
Penicillium griseofulvum
Metab. Eng.
10
246-254
2008
-
2
1
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1
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4
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1
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1
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1
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687164
Puel
The inability of Byssochlamys ...
Byssochlamys nivea, Byssochlamys nivea NRRL 2615
Int. J. Food Microbiol.
115
131-139
2007
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1
1
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7
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671762
Shao
Cloning and characterization o ...
Streptomyces antibioticus
Biochem. Biophys. Res. Commun.
345
133-139
2006
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1
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3
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672715
Wattanachaisaereekul
Optimization of heterologous p ...
Penicillium griseofulvum
Biotechnol. Bioeng.
97
893-900
2006
1
1
1
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1
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6
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673079
Moriguchi
Analysis of subunit interactio ...
Aspergillus terreus
Chembiochem
7
1869-1874
2006
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676009
Lu
A gene (pks2) encoding a putat ...
Glarea lozoyensis
Mol. Genet. Genomics
273
207-216
2005
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486452
Richardson
Tolerance and specificity of r ...
Penicillium griseofulvum
Metab. Eng.
1
180-187
1999
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1
1
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5
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1
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486449
Child
-
Inactivation of the polyketide ...
Penicillium griseofulvum
Biochem. J.
330
933-937
1998
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2
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1
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1
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486451
Campuzano
Incubation of 6-methylsalicyli ...
Penicillium griseofulvum
Biochem. Soc. Trans.
26
S284
1998
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1
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9
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9
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486450
Child
Structural similarities betwee ...
Penicillium griseofulvum
Biochemistry
35
12267-12274
1996
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486447
Spencer
-
Purification and properties of ...
Penicillium griseofulvum, Penicillium griseofulvum NRRL 2159A
Biochem. J.
288
839-846
1992
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4
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6
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1
1
6
1
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1
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486448
Spencer
Investigation of the mechanism ...
Penicillium griseofulvum
Biochemistry
31
9107-9116
1992
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2
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1
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486446
Lam
In vitro stabilization of 6-me ...
Penicillium griseofulvum, Penicillium griseofulvum NRRL 2159A
Can. J. Microbiol.
34
30-37
1988
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486445
Dimroth
6-Methylsalicylic acid synthet ...
Penicillium griseofulvum, Penicillium griseofulvum NRRL 2159A
Eur. J. Biochem.
68
591-596
1976
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1
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5
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8
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