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show all sequences of 2.3.1.163

Purification and characterization of acetyl coenzyme A: 10-hydroxytaxane O-acetyltransferase from cell suspension cultures of Taxus chinensis

Menhard, B.; Zenk, M.H.; Phytochemistry 50, 763-774 (1999)

Data extracted from this reference:

Application
Application
Commentary
Organism
pharmacology
use in synthesis of taxol for anticnacer treatment
Taxus cuspidata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
acetyl-CoA
-
Taxus cuspidata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70900
-
1 * 70900, SDS-PAGE
Taxus cuspidata
71000
-
gel filtration
Taxus cuspidata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-desacetyltaxuyunnanin C
Taxus cuspidata
-
CoA + taxuyunnanin C
-
Taxus cuspidata
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus cuspidata
-
-
-
Purification (Commentary)
Commentary
Organism
-
Taxus cuspidata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5,10,14-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
CoA + 2,5,10-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
?
acetyl-CoA + 10-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
CoA + taxuyunnanin C
-
486444
Taxus cuspidata
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 70900, SDS-PAGE
Taxus cuspidata
Application (protein specific)
Application
Commentary
Organism
pharmacology
use in synthesis of taxol for anticnacer treatment
Taxus cuspidata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
acetyl-CoA
-
Taxus cuspidata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70900
-
1 * 70900, SDS-PAGE
Taxus cuspidata
71000
-
gel filtration
Taxus cuspidata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 10-desacetyltaxuyunnanin C
Taxus cuspidata
-
CoA + taxuyunnanin C
-
Taxus cuspidata
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Taxus cuspidata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5,10,14-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
CoA + 2,5,10-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
?
acetyl-CoA + 10-desacetyltaxuyunnanin C
-
486444
Taxus cuspidata
CoA + taxuyunnanin C
-
486444
Taxus cuspidata
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 70900, SDS-PAGE
Taxus cuspidata
Other publictions for EC 2.3.1.163
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486444
Menhard
Purification and characterizat ...
Taxus cuspidata
Phytochemistry
50
763-774
1999
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