BRENDA - Enzyme Database show
show all sequences of 2.3.1.161

Complete reconstitution of a highly reducing iterative polyketide synthase

Ma, S.M.; Li, J.W.; Choi, J.W.; Zhou, H.; Lee, K.K.; Moorthie, V.A.; Xie, X.; Kealey, J.T.; Da Silva, N.A.; Vederas, J.C.; Tang, Y.; Science 326, 589-592 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Aspergillus terreus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus terreus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus terreus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+
accessory protein LovC is needed: Loading of the megasynthase by malonyl-CoA is presumably followed by decarboxylation to yield the acetyl starter unit. Each round of Claisen condensation is catalyzed by the KS domain, whereas the growing polyketide is tethered to the phosphopantetheinyl arm of the ACP. After each condensation, the polyketide is subjected to a different combination of tailoring, which can include alpha-methylation by the MT domain, beta-ketoreduction by the KR domain, beta-dehydration by the DH domain, and alpha-beta-enoylreduction by the dissociated LovC. The different tailoring permutations after each round of chain extension yield a triene-containing hexaketide thioester that can undergo a stereospecific Diels-Alder cyclization to yield the decalin portion. After formation of the nonaketide, the chain is released to yield the ring-open form. In the absence of LovC, methylated hexa- and heptaketide pyrones are the primary products.
706764
Aspergillus terreus
dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
-
-
-
ir
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus terreus
S-adenosyl-L-methionine
-
Aspergillus terreus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Aspergillus terreus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus terreus
S-adenosyl-L-methionine
-
Aspergillus terreus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+
accessory protein LovC is needed: Loading of the megasynthase by malonyl-CoA is presumably followed by decarboxylation to yield the acetyl starter unit. Each round of Claisen condensation is catalyzed by the KS domain, whereas the growing polyketide is tethered to the phosphopantetheinyl arm of the ACP. After each condensation, the polyketide is subjected to a different combination of tailoring, which can include alpha-methylation by the MT domain, beta-ketoreduction by the KR domain, beta-dehydration by the DH domain, and alpha-beta-enoylreduction by the dissociated LovC. The different tailoring permutations after each round of chain extension yield a triene-containing hexaketide thioester that can undergo a stereospecific Diels-Alder cyclization to yield the decalin portion. After formation of the nonaketide, the chain is released to yield the ring-open form. In the absence of LovC, methylated hexa- and heptaketide pyrones are the primary products.
706764
Aspergillus terreus
dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
-
-
-
ir
Other publictions for EC 2.3.1.161
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736314
Cacho
Understanding programming of f ...
Aspergillus terreus
J. Am. Chem. Soc.
137
15688-15691
2015
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735434
Xu
LovG: The thioesterase require ...
Aspergillus terreus
Angew. Chem. Int. Ed. Engl.
52
6472-6475
2013
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701673
Lee
Determination of the extent of ...
Aspergillus terreus
Anal. Biochem.
394
75-80
2009
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706764
Ma
Complete reconstitution of a h ...
Aspergillus terreus
Science
326
589-592
2009
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673594
Ma
Biochemical characterization o ...
Aspergillus terreus
FEBS J.
274
2854-2864
2007
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676287
Burr
Syntheses of conjugated pyrone ...
Aspergillus terreus
Org. Lett.
9
161-164
2007
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660049
Sorensen
Transformations of cyclic nona ...
Aspergillus terreus
Org. Biomol. Chem.
1
50-59
2003
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441503
Auclair
-
Lovastatin nonaketide synthase ...
Aspergillus terreus
J. Am. Chem. Soc.
122
11519-11520
2000
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1
1
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441505
Hutchinson
Aspects of the biosynthesis of ...
Aspergillus terreus
Antonie van Leeuwenhoek
78
287-295
2000
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441504
Hendrickson
Lovastatin biosynthesis in Asp ...
Aspergillus terreus
Chem. Biol.
6
429-439
1999
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441506
Kennedy
Modulation of polyketide synth ...
Aspergillus terreus
Science
284
1368-1372
1999
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1
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