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show all sequences of 2.3.1.161

Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB

Ma, S.M.; Tang, Y.; FEBS J. 274, 2854-2864 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
minimal polyketide synthase domains of subunit LovB as standalone proteins; the entire lovB gene is inserted into pET21a+ to create pSMa33, the LovB KS-MAT didomain, MAT, ACP, ACP-CON and CON are each amplified from pSMa33 and cloned into pET28a+
Aspergillus terreus
Engineering
Amino acid exchange
Commentary
Organism
additional information
expression of minimal polyketide synthase domains of subunit LovB as standalone proteins. The didomain proteins ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase KS-MAT can transfer the acyl group to both the cognate LovB acyl carrier protein and heterologous acyl carrier proteins from bacterial type I and type II polyketide synthases. It transfers malonyl and acetyl groups with kcat/Km values of 0.62 per min and 0.032 per min, resp
Aspergillus terreus
S208A
LovB MAT mutant
Aspergillus terreus
S209A
LovB MAT mutant
Aspergillus terreus
S656A
inactivation of malonyl-coenzyme A:acyl carrier protein acyltransferase domain in ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase for assay of ketosynthase activity. Ketosynthase displays no activity towards acetyl groups but recognizes malonyl groups in the absence of cerulenin; LovB KS-MAT mutant, MAT domain inactivated
Aspergillus terreus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
LovB ACP, determined by SDS-PAGE
Aspergillus terreus
52000
-
LovB MAT, determined by SDS-PAGE
Aspergillus terreus
66000
-
LovB ACP-CON, determined by SDS-PAGE
Aspergillus terreus
103000
-
LovB KS-MAT, determined by SDS-PAGE
Aspergillus terreus
335000
-
LovB
Aspergillus terreus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
Aspergillus terreus
-
dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus
Q9Y8A5
-
-
Purification (Commentary)
Commentary
Organism
the single domains are purified using Ni-nitrilotriacetic agarose resin and anion-exchange chromatography
Aspergillus terreus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
-
673594
Aspergillus terreus
dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
minimal polyketide synthase domains of subunit LovB as standalone proteins
Aspergillus terreus
the entire lovB gene is inserted into pET21a+ to create pSMa33, the LovB KS-MAT didomain, MAT, ACP, ACP-CON and CON are each amplified from pSMa33 and cloned into pET28a+
Aspergillus terreus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
expression of minimal polyketide synthase domains of subunit LovB as standalone proteins. The didomain proteins ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase KS-MAT can transfer the acyl group to both the cognate LovB acyl carrier protein and heterologous acyl carrier proteins from bacterial type I and type II polyketide synthases. It transfers malonyl and acetyl groups with kcat/Km values of 0.62 per min and 0.032 per min, resp
Aspergillus terreus
S208A
LovB MAT mutant
Aspergillus terreus
S209A
LovB MAT mutant
Aspergillus terreus
S656A
inactivation of malonyl-coenzyme A:acyl carrier protein acyltransferase domain in ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase for assay of ketosynthase activity. Ketosynthase displays no activity towards acetyl groups but recognizes malonyl groups in the absence of cerulenin
Aspergillus terreus
S656A
LovB KS-MAT mutant, MAT domain inactivated
Aspergillus terreus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
LovB ACP, determined by SDS-PAGE
Aspergillus terreus
52000
-
LovB MAT, determined by SDS-PAGE
Aspergillus terreus
66000
-
LovB ACP-CON, determined by SDS-PAGE
Aspergillus terreus
103000
-
LovB KS-MAT, determined by SDS-PAGE
Aspergillus terreus
335000
-
LovB
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
Aspergillus terreus
-
dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
the single domains are purified using Ni-nitrilotriacetic agarose resin and anion-exchange chromatography
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
-
673594
Aspergillus terreus
dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
-
-
?
Other publictions for EC 2.3.1.161
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Biochemical characterization o ...
Aspergillus terreus
FEBS J.
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2007
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Aspergillus terreus
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441503
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Lovastatin nonaketide synthase ...
Aspergillus terreus
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122
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441504
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Science
284
1368-1372
1999
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