BRENDA - Enzyme Database show
show all sequences of 2.3.1.160

Crystal structure of vinorine synthase, the first representative of the BAHD superfamily

Ma, X.; Koepke, J.; Panjikar, S.; Fritzsch, G.; Stockigt, J.; J. Biol. Chem. 280, 13576-13583 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression of N-terminally His-tagged enzyme in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary)
Crystallization
Organism
purified detagged recombinant wild-type or selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 32C, 2-3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM acetyl-CoA, with reservoir solution containing 0.1 M Tris-HCl, pH 8.7, 2 M ammonium sulfate, 2% PEG 400, cryoprotection by 20-25% glycerol, X-ray diffraction structure determination anad analysis at 2.6 A resolution, modeling of CoA binding
Rauvolfia serpentina
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 16-epivellosimine
Rauvolfia serpentina
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
CoA + vinorine
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rauvolfia serpentina
Q70PR7
-
-
Purification (Commentary)
Commentary
Organism
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
Rauvolfia serpentina
Reaction
Reaction
Commentary
Organism
acetyl-CoA + 16-epivellosimine = CoA + vinorine
His160 of the HxxxD motif functions as a general base in catalysis, located at the center of the reaction channel at the interface of both domains and is accessible from both sides
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 16-epivellosimine
-
662350
Rauvolfia serpentina
CoA + vinorine
-
-
-
r
acetyl-CoA + 16-epivellosimine
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
662350
Rauvolfia serpentina
CoA + vinorine
-
-
-
r
Subunits
Subunits
Commentary
Organism
monomer
two-domain structure
Rauvolfia serpentina
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of N-terminally His-tagged enzyme in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified detagged recombinant wild-type or selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 32C, 2-3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM acetyl-CoA, with reservoir solution containing 0.1 M Tris-HCl, pH 8.7, 2 M ammonium sulfate, 2% PEG 400, cryoprotection by 20-25% glycerol, X-ray diffraction structure determination anad analysis at 2.6 A resolution, modeling of CoA binding
Rauvolfia serpentina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 16-epivellosimine
Rauvolfia serpentina
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
CoA + vinorine
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 16-epivellosimine
-
662350
Rauvolfia serpentina
CoA + vinorine
-
-
-
r
acetyl-CoA + 16-epivellosimine
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
662350
Rauvolfia serpentina
CoA + vinorine
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
two-domain structure
Rauvolfia serpentina
Other publictions for EC 2.3.1.160
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
682286
Stoeckigt
-
The molecular architecture of ...
Rauvolfia serpentina
Phytochem. Rev.
6
15-34
2007
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-
1
1
5
-
-
-
-
-
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-
-
1
-
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1
-
-
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1
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-
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1
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1
5
-
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-
-
-
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-
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662350
Ma
Crystal structure of vinorine ...
Rauvolfia serpentina
J. Biol. Chem.
280
13576-13583
2005
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-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
-
-
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-
1
-
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-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661320
Gerasimenko
Purification and partial amino ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2781-2786
2004
-
-
-
-
-
-
-
2
-
-
2
1
-
5
-
-
1
-
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
2
-
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2
1
-
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1
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
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-
661321
Bayer
Acetyltransfer in natural prod ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2787-2795
2004
-
-
1
-
13
-
7
9
-
-
1
1
-
7
-
-
1
1
-
-
-
-
4
1
1
-
-
7
1
-
-
-
-
1
-
-
-
1
-
-
13
-
-
7
-
9
-
-
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
7
1
-
-
1
-
-
-
-
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-
486439
Pfitzner
-
Properties of vinorine synthas ...
Rauvolfia serpentina
Z. Naturforsch. C
41
103-114
1986
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-
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-
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3
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1
1
-
1
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1
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1
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2
-
1
1
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1
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-
-
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-
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3
-
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1
1
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1
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1
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2
-
1
1
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1
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-
-
-
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-
486440
Pfitzner
-
Biogenetic link between sarpag ...
Rauvolfia serpentina
Tetrahedron Lett.
24
5197-5200
1983
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-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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