BRENDA - Enzyme Database show
show all sequences of 2.3.1.160

Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis

Gerasimenko, I.; Ma, X.; Sheludko, Y.; Mentele, R.; Lottspeich, F.; Stockigt, J.; Bioorg. Med. Chem. 12, 2781-2786 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0075
-
gardneral
pH 7.0, 30°C
Rauvolfia serpentina x Rhazya stricta
0.057
-
acetyl-CoA
pH 7.0, 30°C
Rauvolfia serpentina x Rhazya stricta
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
about, gel filtration
Rauvolfia serpentina x Rhazya stricta
50000
-
1 * 50000, about, SDS-PAGE
Rauvolfia serpentina x Rhazya stricta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 16-epivellosimine
Rauvolfia serpentina x Rhazya stricta
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
CoA + vinorine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rauvolfia serpentina x Rhazya stricta
-
hybrid
-
Purification (Commentary)
Commentary
Organism
991fold from cell suspension culture by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, and gel filtration
Rauvolfia serpentina x Rhazya stricta
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
hybrid of Rauvolfia serpentina and Rhazya stricta
Rauvolfia serpentina x Rhazya stricta
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
99.1
-
purified enzyme
Rauvolfia serpentina x Rhazya stricta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 16-epivellosimine
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
661320
Rauvolfia serpentina x Rhazya stricta
CoA + vinorine
-
-
-
?
acetyl-CoA + 16-epivellosimine
the forward reaction is preferred
661320
Rauvolfia serpentina x Rhazya stricta
CoA + vinorine
-
-
-
r
acetyl-CoA + gardneral
sarpagan-type alkaloid, the forward reaction is preferred
661320
Rauvolfia serpentina x Rhazya stricta
CoA + 11-methoxyvinorine
ajmalin-type alkaloid
-
-
r
additional information
the acetylation is important for stabilization of the immediate indolenine 17-deacetyl-vinorine, which would otherwise be spontaneously re-transformed
661320
Rauvolfia serpentina x Rhazya stricta
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 50000, about, SDS-PAGE
Rauvolfia serpentina x Rhazya stricta
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rauvolfia serpentina x Rhazya stricta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Rauvolfia serpentina x Rhazya stricta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0075
-
gardneral
pH 7.0, 30°C
Rauvolfia serpentina x Rhazya stricta
0.057
-
acetyl-CoA
pH 7.0, 30°C
Rauvolfia serpentina x Rhazya stricta
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
about, gel filtration
Rauvolfia serpentina x Rhazya stricta
50000
-
1 * 50000, about, SDS-PAGE
Rauvolfia serpentina x Rhazya stricta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + 16-epivellosimine
Rauvolfia serpentina x Rhazya stricta
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
CoA + vinorine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
991fold from cell suspension culture by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, and gel filtration
Rauvolfia serpentina x Rhazya stricta
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
hybrid of Rauvolfia serpentina and Rhazya stricta
Rauvolfia serpentina x Rhazya stricta
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
99.1
-
purified enzyme
Rauvolfia serpentina x Rhazya stricta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 16-epivellosimine
the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview
661320
Rauvolfia serpentina x Rhazya stricta
CoA + vinorine
-
-
-
?
acetyl-CoA + 16-epivellosimine
the forward reaction is preferred
661320
Rauvolfia serpentina x Rhazya stricta
CoA + vinorine
-
-
-
r
acetyl-CoA + gardneral
sarpagan-type alkaloid, the forward reaction is preferred
661320
Rauvolfia serpentina x Rhazya stricta
CoA + 11-methoxyvinorine
ajmalin-type alkaloid
-
-
r
additional information
the acetylation is important for stabilization of the immediate indolenine 17-deacetyl-vinorine, which would otherwise be spontaneously re-transformed
661320
Rauvolfia serpentina x Rhazya stricta
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50000, about, SDS-PAGE
Rauvolfia serpentina x Rhazya stricta
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rauvolfia serpentina x Rhazya stricta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Rauvolfia serpentina x Rhazya stricta
Other publictions for EC 2.3.1.160
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
682286
Stoeckigt
-
The molecular architecture of ...
Rauvolfia serpentina
Phytochem. Rev.
6
15-34
2007
-
-
1
1
5
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662350
Ma
Crystal structure of vinorine ...
Rauvolfia serpentina
J. Biol. Chem.
280
13576-13583
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661320
Gerasimenko
Purification and partial amino ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2781-2786
2004
-
-
-
-
-
-
-
2
-
-
2
1
-
5
-
-
1
-
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661321
Bayer
Acetyltransfer in natural prod ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2787-2795
2004
-
-
1
-
13
-
7
9
-
-
1
1
-
7
-
-
1
1
-
-
-
-
4
1
1
-
-
7
1
-
-
-
-
1
-
-
-
1
-
-
13
-
-
7
-
9
-
-
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
7
1
-
-
1
-
-
-
-
-
-
486439
Pfitzner
-
Properties of vinorine synthas ...
Rauvolfia serpentina
Z. Naturforsch. C
41
103-114
1986
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
2
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
486440
Pfitzner
-
Biogenetic link between sarpag ...
Rauvolfia serpentina
Tetrahedron Lett.
24
5197-5200
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-