Cloned (Comment) | Organism |
---|---|
His-tagged wild-type and His352 mutant proteins overexpressed in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H352A | 3.3fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1154 times | Rattus norvegicus |
H352E | 2.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1250 times | Rattus norvegicus |
H352K | 3.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 526 times | Rattus norvegicus |
H352Y | 3.1fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 429 times | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
acetoacetyl-CoA | pH 8.2, wild-type enzyme | Rattus norvegicus | |
0.058 | - |
acetoacetyl-CoA | pH 8.2, mutant enzyme H352E | Rattus norvegicus | |
0.065 | - |
acetoacetyl-CoA | pH 8.2, mutant enzyme H352Y | Rattus norvegicus | |
0.07 | - |
acetoacetyl-CoA | pH 8.2, mutant enzyme H352A | Rattus norvegicus | |
0.079 | - |
acetoacetyl-CoA | pH 8.2, mutant enzyme H352K | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | key enzyme involved in fatty acid oxidation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, wild-type and mutant enzymes H352E, H352A, H352K and H352Y, stable for at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Rattus norvegicus | acetyl-CoA + acetyl-CoA | - |
? | |
additional information | key enzyme involved in fatty acid oxidation | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-CoA thiolase | - |
Rattus norvegicus |