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Literature summary for 2.3.1.16 extracted from
- Glyoxysomal acetoacetyl-CoA thiolase and 3-oxoacyl-CoA thiolase from sunflower cotyledons (2002), Planta, 214, 597-607.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| palmitoyl-CoA | up to 0.03 mM | Helianthus annuus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values between 0.003 and 0.007 mM with substrates having a carbon chain length from 6 to 16 carbon atoms, Km values for CoA at high and low acetoacetyl-CoA concentration | Helianthus annuus | |
| 0.027 | - |
acetoacetyl-CoA | - |
Helianthus annuus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glyoxysome | - |
Helianthus annuus | 9514 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Helianthus annuus |
| 75000 | - |
gel filtration | Helianthus annuus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| achene | - |
Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 352 | - |
substrate: acetoacetyl-CoA | Helianthus annuus |
| 1988 | - |
substrate: 3-oxohexadecanoyl-CoA | Helianthus annuus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + 3-oxoacyl-CoA | - |
Helianthus annuus | acyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | - |
Helianthus annuus | acetyl-CoA + acetyl-CoA | - |
? | |
| additional information | low activity with butyryl-CoA | Helianthus annuus | ? | - |
? | |
| additional information | activity of the enzyme towards 3-oxoacy-CoAs increased with increasing chain length of the substrate | Helianthus annuus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Helianthus annuus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Helianthus annuus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
pI: 8.0 | Helianthus annuus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5 and 9.5: 50% maximum activity, pH 6.5: no activity | Helianthus annuus |
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Release 2023.1 (January 2023)
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