General Stability | Organism |
---|---|
inactivation at Tris-HCl concentration | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.031 | - |
acetoacetyl-CoA | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
multienzyme complex: enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase, PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + 3-oxoacyl-CoA | Escherichia coli | - |
acyl-CoA + acetyl-CoA | - |
? | |
CoA + 3-oxoacyl-CoA | Escherichia coli B / ATCC 11303 | - |
acyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA | multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase : EC 5.1.2.3 and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase: EC 5.3.3.3 | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:long-chain fatty acid-grown cell | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-76°C, 0.2 M potassium phosphate, pH 6.6, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + 3-oxoacyl-CoA | - |
Escherichia coli | acyl-CoA + acetyl-CoA | - |
? | |
CoA + 3-oxoacyl-CoA | - |
Escherichia coli | acyl-CoA + acetyl-CoA | - |
r | |
CoA + 3-oxoacyl-CoA | - |
Escherichia coli B / ATCC 11303 | acyl-CoA + acetyl-CoA | - |
? | |
CoA + 3-oxoacyl-CoA | - |
Escherichia coli B / ATCC 11303 | acyl-CoA + acetyl-CoA | - |
r | |
CoA + acetoacetyl-CoA | - |
Escherichia coli | acetyl-CoA + acetyl-CoA | - |
r | |
additional information | highest activities with medium-chain substrates | Escherichia coli | ? | - |
? | |
additional information | highest activities with medium-chain substrates | Escherichia coli B / ATCC 11303 | ? | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
multienzyme complex, -76°C, 25% glycerol v/v, stable for months | Escherichia coli |