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Literature summary for 2.3.1.16 extracted from

  • Binstock, J.F.; Schulz, H.
    Fatty acid oxidation complex from Escherichia coli (1981), Methods Enzymol., 71, 403-411.
    View publication on PubMed

General Stability

General Stability Organism
inactivation at Tris-HCl concentration Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.031
-
acetoacetyl-CoA
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
multienzyme complex: enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase, PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + 3-oxoacyl-CoA Escherichia coli
-
acyl-CoA + acetyl-CoA
-
?
CoA + 3-oxoacyl-CoA Escherichia coli B / ATCC 11303
-
acyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase : EC 5.1.2.3 and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase: EC 5.3.3.3 Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:long-chain fatty acid-grown cell
-
Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-76°C, 0.2 M potassium phosphate, pH 6.6, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + 3-oxoacyl-CoA
-
Escherichia coli acyl-CoA + acetyl-CoA
-
?
CoA + 3-oxoacyl-CoA
-
Escherichia coli acyl-CoA + acetyl-CoA
-
r
CoA + 3-oxoacyl-CoA
-
Escherichia coli B / ATCC 11303 acyl-CoA + acetyl-CoA
-
?
CoA + 3-oxoacyl-CoA
-
Escherichia coli B / ATCC 11303 acyl-CoA + acetyl-CoA
-
r
CoA + acetoacetyl-CoA
-
Escherichia coli acetyl-CoA + acetyl-CoA
-
r
additional information highest activities with medium-chain substrates Escherichia coli ?
-
?
additional information highest activities with medium-chain substrates Escherichia coli B / ATCC 11303 ?
-
?

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6
-
multienzyme complex, -76°C, 25% glycerol v/v, stable for months Escherichia coli