Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli with an additional hexahistidine tag at the N-terminus | Huperzia serrata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.7 | - |
4-coumaroyl-CoA | pH 7.0, 30°C | Huperzia serrata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45831 | - |
x * 45831, calculated | Huperzia serrata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Huperzia serrata | A3E7Z7 | recombinant enzyme expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3 malonyl-CoA | - |
Huperzia serrata | naringenin chalcone + 4 CoA + 3 CO2 | small amounts of bisnoryangonin and 4-coumaroyltriacetic acid lactone are formed as byproducts | ? | |
4-methoxycinnamoyl-CoA + malonyl-CoA | - |
Huperzia serrata | ? + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-CoA | - |
Huperzia serrata | ? + CoA + CO2 | - |
? | |
benzoyl-CoA + malonyl-CoA | - |
Huperzia serrata | 2,3',4,6-tetrahydroxybenzophenone + CoA + CO2 | - |
? | |
hexanoyl-CoA + malonyl-CoA | - |
Huperzia serrata | ? + CoA + CO2 | - |
? | |
additional information | enzyme accepts bulky substrates as starters and carries out one to three condensations to yield various aromatic tetraketides along with some di- and triketides | Huperzia serrata | ? | - |
? | |
N-methylanthraniloyl-CoA + 3 malonyl-CoA | - |
Huperzia serrata | 1,3-dihydroxy-N-methylacridone + 4 CoA + 3 CO2 | - |
? | |
phenylacetyl-CoA + malonyl-CoA | - |
Huperzia serrata | 2,4,6-trihydroxyphenylbenzylketone + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45831, calculated | Huperzia serrata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
4-coumaroyl-CoA | pH 7.0, 30°C | Huperzia serrata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Huperzia serrata |