BRENDA - Enzyme Database show
show all sequences of 2.3.1.159

Specificities of functionally expressed chalcone and acridone synthases from Ruta graveolens

Springob, K.; Lukacin, R.; Ernwein, C.; Groning, I.; Matern, U.; Eur. J. Biochem. 267, 6552-6559 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence comparison of 4 clones and also with the DNA sequence of Ruta graveolens chalcone synthase
Ruta graveolens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
Ruta graveolens
-
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
Ruta graveolens
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ruta graveolens
-
2 isozymes I and II
-
Reaction
Reaction
Commentary
Organism
3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2
two-step mechanism
Ruta graveolens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ruta graveolens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Ruta graveolens
9.54
-
purified recombinant isozyme I
Ruta graveolens
14.16
-
purified recombinant isozyme II
Ruta graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 4-coumaroyl-CoA
isozyme I shows 16% activity and isozyme II shows 12% activity compared to the activity with N-methylanthraniloyl-CoA as substrate, respectively
486401
Ruta graveolens
CoA + naringenin chalcone
-
486401
Ruta graveolens
?
malonyl-CoA + N-methylanthraniloyl-CoA
-
486401
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
486401
Ruta graveolens
ir
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence comparison of 4 clones and also with the DNA sequence of Ruta graveolens chalcone synthase
Ruta graveolens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
Ruta graveolens
-
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
Ruta graveolens
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ruta graveolens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Ruta graveolens
9.54
-
purified recombinant isozyme I
Ruta graveolens
14.16
-
purified recombinant isozyme II
Ruta graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 4-coumaroyl-CoA
isozyme I shows 16% activity and isozyme II shows 12% activity compared to the activity with N-methylanthraniloyl-CoA as substrate, respectively
486401
Ruta graveolens
CoA + naringenin chalcone
-
486401
Ruta graveolens
?
malonyl-CoA + N-methylanthraniloyl-CoA
-
486401
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
486401
Ruta graveolens
ir
Other publictions for EC 2.3.1.159
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736426
Mori
Cloning and structure-function ...
Citrus x microcarpa
J. Biol. Chem.
288
28845-28858
2013
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4
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3
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3
3
689543
Rohde
Anthranilate N-methyltransfera ...
Ruta graveolens
Plant J.
53
541-553
2008
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3
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1
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673581
Wanibuchi
An acridone-producing novel mu ...
Huperzia serrata
FEBS J.
274
1073-1082
2007
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-
1
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1
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5
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8
1
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1
1
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684180
Morita
Crystallization and preliminar ...
Huperzia serrata
Acta Crystallogr. Sect. F
63
576-578
2007
-
-
1
1
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-
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1
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1
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2
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676366
Lukacin
Starter substrate specificitie ...
Ruta graveolens
Phytochemistry
66
277-284
2005
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1
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1
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1
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1
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486402
Lukacin
Transformation of acridone syn ...
Ruta graveolens
FEBS Lett.
508
413-417
2001
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1
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2
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1
1
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4
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1
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2
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486401
Springob
Specificities of functionally ...
Ruta graveolens
Eur. J. Biochem.
267
6552-6559
2000
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1
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1
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3
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1
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1
3
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486398
Lukacin.R.; Springob
Native acridone synthases I an ...
Ruta graveolens
FEBS Lett.
448
135-140
1999
-
-
1
-
-
-
-
4
-
-
4
1
-
4
-
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3
3
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2
1
2
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-
2
2
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1
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4
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4
1
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3
3
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2
1
2
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2
2
-
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-
486399
Junghanns
Differential regulation and di ...
Ruta graveolens
Phytochemistry
49
403-411
1998
1
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1
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2
-
3
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3
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3
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486400
Junghanns
Molecular cloning and heterolo ...
Ruta graveolens
Plant Mol. Biol.
27
681-692
1995
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1
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2
1
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4
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1
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1
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2
1
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486396
Baumert
Purification and properties of ...
Ruta graveolens
Z. Naturforsch. C
49
26-32
1994
-
-
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-
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3
2
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3
1
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3
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1
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2
1
1
2
1
1
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3
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1
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1
1
2
1
1
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486397
Maier
-
Synthesis of 1,3-dihydroxy-N-m ...
Ruta graveolens
Phytochemistry
32
691-698
1993
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