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Literature summary for 2.3.1.159 extracted from

  • Junghanns, K.T.; Kneusel, R.E.; Baumert, A.; Maier, W.; Groeger, D.; Matern, U.
    Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures (1995), Plant Mol. Biol., 27, 681-692.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence determination Ruta graveolens
expression level in Escherichia coli is decreased by light irradiation and increased by treatment with elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, recombinant enzyme from Escherichia coli, SDS-PAGE and western blot analysis Ruta graveolens
44000
-
recombinant enzyme from E. coli, gel filtration Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens activity is increased upon treatment with crude elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea, syn. Phytophthora sojae, and decreased upon light irradiation CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Ruta graveolens Q9FSC0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Ruta graveolens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA
-
Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
ir
malonyl-CoA + N-methylanthraniloyl-CoA activity is increased upon treatment with crude elicitors from the cell wall of Phytophthora megasperma f. sp. glycinea, syn. Phytophthora sojae, and decreased upon light irradiation Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
ir

Subunits

Subunits Comment Organism
monomer 1 * 42000, recombinant enzyme from Escherichia coli, SDS-PAGE and western blot analysis Ruta graveolens

Synonyms

Synonyms Comment Organism
ACS
-
Ruta graveolens