BRENDA - Enzyme Database show
show all sequences of 2.3.1.159

Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L.

Baumert, A.; Maier, W.; Gröger, D.; Deutzmann, R.; Z. Naturforsch. C 49, 26-32 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
0.5 mM: 95% inhibition
Ruta graveolens
N-ethylmaleimide
1 mM: 100% inhibition
Ruta graveolens
p-chloromercuribenzoate
0.5 mM: 95% inhibition
Ruta graveolens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0106
-
N-methylanthraniloyl-CoA
partially purified enzyme
Ruta graveolens
0.0328
-
malonyl-CoA
partially purified enzyme
Ruta graveolens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid sequence determination
Ruta graveolens
40000
-
2 * 40000, SDS-PAGE
Ruta graveolens
69000
-
gel filtration
Ruta graveolens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
Ruta graveolens
-
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ruta graveolens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Ruta graveolens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ruta graveolens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.035
-
purified enzyme
Ruta graveolens
Storage Stability
Storage Stability
Organism
-70°C, partially purified, stable for at least 4 weeks
Ruta graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
-
486396
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
-
-
?
malonyl-CoA + N-methylanthraniloyl-CoA
-
486396
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
486396
Ruta graveolens
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Ruta graveolens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Ruta graveolens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
0.5 mM: 95% inhibition
Ruta graveolens
N-ethylmaleimide
1 mM: 100% inhibition
Ruta graveolens
p-chloromercuribenzoate
0.5 mM: 95% inhibition
Ruta graveolens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0106
-
N-methylanthraniloyl-CoA
partially purified enzyme
Ruta graveolens
0.0328
-
malonyl-CoA
partially purified enzyme
Ruta graveolens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid sequence determination
Ruta graveolens
40000
-
2 * 40000, SDS-PAGE
Ruta graveolens
69000
-
gel filtration
Ruta graveolens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
Ruta graveolens
-
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ruta graveolens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ruta graveolens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.035
-
purified enzyme
Ruta graveolens
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, partially purified, stable for at least 4 weeks
Ruta graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + N-methylanthraniloyl-CoA
-
486396
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
-
-
?
malonyl-CoA + N-methylanthraniloyl-CoA
-
486396
Ruta graveolens
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
486396
Ruta graveolens
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Ruta graveolens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Ruta graveolens
Other publictions for EC 2.3.1.159
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736426
Mori
Cloning and structure-function ...
Citrus x microcarpa
J. Biol. Chem.
288
28845-28858
2013
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1
1
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4
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3
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3
3
689543
Rohde
Anthranilate N-methyltransfera ...
Ruta graveolens
Plant J.
53
541-553
2008
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-
-
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3
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1
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673581
Wanibuchi
An acridone-producing novel mu ...
Huperzia serrata
FEBS J.
274
1073-1082
2007
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1
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5
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8
1
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1
1
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684180
Morita
Crystallization and preliminar ...
Huperzia serrata
Acta Crystallogr. Sect. F
63
576-578
2007
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-
1
1
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1
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676366
Lukacin
Starter substrate specificitie ...
Ruta graveolens
Phytochemistry
66
277-284
2005
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-
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1
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1
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1
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1
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486402
Lukacin
Transformation of acridone syn ...
Ruta graveolens
FEBS Lett.
508
413-417
2001
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1
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2
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1
1
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4
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1
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2
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486401
Springob
Specificities of functionally ...
Ruta graveolens
Eur. J. Biochem.
267
6552-6559
2000
-
-
1
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1
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3
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1
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1
3
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2
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1
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3
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2
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486398
Lukacin.R.; Springob
Native acridone synthases I an ...
Ruta graveolens
FEBS Lett.
448
135-140
1999
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1
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4
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4
1
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4
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3
3
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2
1
2
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2
2
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1
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4
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4
1
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3
3
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2
1
2
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2
2
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486399
Junghanns
Differential regulation and di ...
Ruta graveolens
Phytochemistry
49
403-411
1998
1
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1
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3
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486400
Junghanns
Molecular cloning and heterolo ...
Ruta graveolens
Plant Mol. Biol.
27
681-692
1995
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1
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2
1
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4
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2
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2
1
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1
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2
1
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2
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2
1
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486396
Baumert
Purification and properties of ...
Ruta graveolens
Z. Naturforsch. C
49
26-32
1994
-
-
-
-
-
-
3
2
-
-
3
1
-
3
-
-
1
-
-
2
1
1
2
1
1
-
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3
-
2
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3
1
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1
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2
1
1
2
1
1
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486397
Maier
-
Synthesis of 1,3-dihydroxy-N-m ...
Ruta graveolens
Phytochemistry
32
691-698
1993
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