Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method at 22°C, crystallization of the Y97N protein | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
T80S/Y97N | the mutant enzyme shows 6.5times-higher activity, compared to that of the wild-type ST0452 protein, revealing that these two substituted residues function cooperatively to increase N-acetylglucosamine-1-phosphate uridyltransferase activity | Sulfurisphaera tokodaii |
Y97N | the mutant enzyme exhibits over 4 times higher N-acetylglucosamine-1-phosphate uridyltransferase activity, compared with that of the wild-type ST0452 protein. The three-dimensional structure of the Y97N protein is not changed by this substitution but the interactions with the substrate are slightly modified, which might cause the activity to increase. The crystal structure of the Y97N protein shows that positions 146 (Glu) and 80 (Thr) form interactions with GlcNAc, and an engineering strategy is applied to these residues to increase activity | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q975F9 | - |
- |
Sulfurisphaera tokodaii DSM 16993 | Q975F9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ST0452 | - |
Sulfurisphaera tokodaii |