Literature summary for 2.3.1.157 extracted from

Patin, D.; Bayliss, M.; Mengin-Lecreulx, D.; Oyston, P.; Blanot, D.
Purification and biochemical characterisation of GlmU from Yersinia pestis (2015), Arch. Microbiol., 197, 371-378.

Search for more literature for 2.3.1.157

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	presence required	Yersinia pestis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Yersinia pestis
gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Yersinia pseudotuberculosis

Protein Variants

Protein Variants	Comment	Organism
C307S	site-directed mutagenesis	Yersinia pseudotuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
2-nitro-5-thiocyanatobenzoic acid	0.5 mM, 5% residual activity	Yersinia pestis
5,5'-dithiobis-(2-nitrobenzoic acid)	0.1 mM, 33% residual activity	Yersinia pestis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.3	-	alpha-D-glucosamine 1-phosphate	pH 8.2, 37°C	Yersinia pestis
0.3	-	alpha-D-glucosamine 1-phosphate	pH 8.2, 37°C, recombinant His6-tagged enzyme	Yersinia pseudotuberculosis
0.52	-	acetyl-CoA	pH 8.2, 37°C	Yersinia pestis
0.52	-	acetyl-CoA	pH 8.2, 37°C, recombinant His6-tagged enzyme	Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Yersinia pseudotuberculosis
Mg2+	required for both UDP-N-acetylglucosamine pyrophosphorylase and glucosamine-1-phosphate N-acetyltransferase activities, optimum concentration 5 mM	Yersinia pestis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52965	-	MALDI-TOF MS, SDS-PAGE	Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate	Yersinia pseudotuberculosis	-	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?
acetyl-CoA + alpha-D-glucosamine 1-phosphate	Yersinia pseudotuberculosis YPIII	-	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Yersinia pestis	-	-	-
Yersinia pestis YPIII	-	-	-
Yersinia pseudotuberculosis	-	-	-
Yersinia pseudotuberculosis YPIII	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate	-	Yersinia pseudotuberculosis	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?
acetyl-CoA + alpha-D-glucosamine 1-phosphate	-	Yersinia pestis	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?
acetyl-CoA + alpha-D-glucosamine 1-phosphate	-	Yersinia pseudotuberculosis YPIII	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?
acetyl-CoA + alpha-D-glucosamine 1-phosphate	-	Yersinia pestis YPIII	CoA + N-acetyl-alpha-D-glucosamine 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 52965, recombinant His6-tagged enzyme, SDS-PAGE and mass spectrometry	Yersinia pseudotuberculosis
More	C324 does not form a disulphide bond with any other cysteine. This is consistent with the replacement of C307 by a serine in Y. pestis and the great distance between the three other cysteines (C296, C301 and C385) and C324	Yersinia pseudotuberculosis

Synonyms

Synonyms	Comment	Organism
bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase	-	Yersinia pestis
GlcN-1-P acetyltransferase	-	Yersinia pseudotuberculosis
GlmU	-	Yersinia pseudotuberculosis
GlmU	-	Yersinia pestis
UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase	-	Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Yersinia pseudotuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
828	-	alpha-D-glucosamine 1-phosphate	pH 8.2, 37°C	Yersinia pestis
828	-	alpha-D-glucosamine 1-phosphate	pH 8.2, 37°C, recombinant His6-tagged enzyme	Yersinia pseudotuberculosis
853	-	acetyl-CoA	pH 8.2, 37°C	Yersinia pestis
853	-	acetyl-CoA	pH 8.2, 37°C, recombinant His6-tagged enzyme	Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	-	assay at	Yersinia pseudotuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Yersinia pseudotuberculosis

General Information

General Information	Comment	Organism
evolution	the GlmU proteins encoded by Yersinia pestis and Yersinia pseudotuberculosis are identical in amino acid sequence	Yersinia pseudotuberculosis
physiological function	the bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase enzyme GlmU is an essential gene in Yersinia	Yersinia pseudotuberculosis

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Release 2023.1 (January 2023)