Cloned (Comment) | Organism |
---|---|
gene glmU, expression of N-terminally His-tagged wild-type GlmU and mutant GlmUs in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
E458A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, 86% of wild-type acetyltransferase activity is remaining | Mycobacterium tuberculosis |
H374A | site-directed mutagenesis, the enzyme activity of the mutant is abolished by more than 90% of the wild-type acetyltransferase, and the affinity with the two substrates is completely lost | Mycobacterium tuberculosis |
K362A | site-directed mutagenesis, the enzyme activity of the mutant is abolished by more than 90% of the wild-type acetyltransferase, and the affinity with the two substrates is completely lost | Mycobacterium tuberculosis |
K403A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, 27% of wild-type acetyltransferase activity is remaining | Mycobacterium tuberculosis |
N456A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme, 67% of wild-type acetyltransferase activity is remaining | Mycobacterium tuberculosis |
R463A | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Mycobacterium tuberculosis |
S416A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, 65% of wild-type acetyltransferase activity is remaining | Mycobacterium tuberculosis |
S474A | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Mycobacterium tuberculosis |
W460A | site-directed mutagenesis, the enzyme activity of the mutant is abolished by more than 90% of the wild-type acetyltransferase, and the affinity with the two substrates is completely lost | Mycobacterium tuberculosis |
Y398A | site-directed mutagenesis, the enzyme activity of the mutant is abolished by more than 90% of the wild-type acetyltransferase, and the affinity with the two substrates is completely lost | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant K403A | Mycobacterium tuberculosis | |
0.044 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant N456A | Mycobacterium tuberculosis | |
0.056 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant K403A | Mycobacterium tuberculosis | |
0.06 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant E458A | Mycobacterium tuberculosis | |
0.061 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged wild-type GlmU | Mycobacterium tuberculosis | |
0.066 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant E458A | Mycobacterium tuberculosis | |
0.069 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant R463A | Mycobacterium tuberculosis | |
0.07 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S416A | Mycobacterium tuberculosis | |
0.073 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S474A | Mycobacterium tuberculosis | |
0.13 | - |
alpha-D-glucosamine 1-phosphate | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S416A | Mycobacterium tuberculosis | |
0.224 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged wild-type GlmU | Mycobacterium tuberculosis | |
0.242 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S474A | Mycobacterium tuberculosis | |
0.304 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant R463A | Mycobacterium tuberculosis | |
0.343 | - |
acetyl-CoA | pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant N456A | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54100 | - |
x * 54100,, recombinant Histagged GlmU, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene glmU | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type GlmU and mutant GlmUs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54100,, recombinant Histagged GlmU, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GlmU | - |
Mycobacterium tuberculosis |
GlmU acetyltransferase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | GlmU is a bifunctional enzyme with acetyltransferase activity in C-terminus, residues 251-495, and uridyltransferase activity in N-terminus, and it is involved in the biosynthesis of glycosyl donor UDP-N-acetylglucosamine | Mycobacterium tuberculosis |