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Literature summary for 2.3.1.153 extracted from

  • Fujiwara, H.; Tanaka, Y.; Fukui, Y.; Nakao, M.; Ashikari, T.; Kusumi, T.
    Anthocyanin 5-aromatic acyltransferase from Gentiana triflora (1997), Eur. J. Biochem., 249, 45-51.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
PCMB 90% inhibition at 0.1 mM, complete inhibition at 1 mM Gentiana triflora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
caffeoyl-CoA with delphinidin 3,5-diglucoside as cosubstrate Gentiana triflora
0.19
-
p-Coumaroyl-CoA with delphinidin 3,5-diglucoside as cosubstrate Gentiana triflora
0.15
-
delphinidin 3,5-diglucoside with caffeoyl-CoA as cosubstrate Gentiana triflora
0.087
-
cyanidin 3,5-diglucoside with caffeoyl-CoA as cosubstrate Gentiana triflora

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ strongly enhances activity Gentiana triflora
Zn2+ moderately enhances activity Gentiana triflora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
1 * 52000, SDS-PAGE Gentiana triflora
49000
-
gel filtration Gentiana triflora

Organism

Organism UniProt Comment Textmining
Gentiana triflora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gentiana triflora

Source Tissue

Source Tissue Comment Organism Textmining
petal blue Gentiana triflora
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gentiana triflora

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris/HCl, pH 7.0, 0.01 mM (p-aminidinophenyl)methanesulfonyl fluoride, 1 mM DTT, stable for at least 2 years Gentiana triflora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delphinidin 3,5-di-O-beta-D-glucoside + caffeoyl-CoA
-
Gentiana triflora CoA + delphinidin 3-O-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
?
cyanidin-3,5-O-beta-D-diglucoside + caffeoyl-CoA
-
Gentiana triflora CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-caffeoyl)-beta-D-glucoside
-
?
delphinidin 3,5-di-O-beta-D-glucoside + 4-coumaroyl-CoA
-
Gentiana triflora CoA + delphinidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
?
cyanidin-3,5-O-beta-D-diglucoside + 4-coumaroyl-CoA
-
Gentiana triflora CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Gentiana triflora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Gentiana triflora

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 active in this range Gentiana triflora