Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of C-terminally His-tagged BPS in Escherichia coli | Garcinia mangostana |
Protein Variants | Comment | Organism |
---|---|---|
A257G | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
G339S | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
G339V | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
T133L | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00963 | - |
benzoyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
0.01638 | - |
malonyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
45618 | - |
2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
88000 | - |
His-tagged enzyme, gel filtration | Garcinia mangostana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Garcinia mangostana | L7NCQ3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged BPS from Escherichia coli | Garcinia mangostana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pericarp | expression of GmBPS in fruit pericarp from an early stage | Garcinia mangostana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 malonyl-CoA + 4-coumaroyl-CoA | - |
Garcinia mangostana | 3 CoA + 4-hydroxy-6-[(E)-2-(4-hydroxyphenyl)ethenyl]-2H-pyran-2-one + 2 CO2 | - |
? | |
3 malonyl-CoA + acetyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
3 malonyl-CoA + benzoyl-CoA | - |
Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 4-hydroxy-6-(2-oxo-2-phenylethyl)-2H-pyran-2-one + 4-hydroxy-6-phenyl-2H-pyran-2-one + 3 CO2 | the recombinant enzyme produces 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate but also lactones of tetraketide and triketide type | ? | |
3 malonyl-CoA + cinnamoyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-[(E)-2-phenylethenyl]-2H-pyran-2-one + 3 CO2 | - |
? | |
3 malonyl-CoA + hexanoyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-pentyl-2H-pyran-2-one + 5-hexanoyl-4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
3 malonyl-CoA + phenylacetyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-(2-oxo-3-phenylpropyl)-2H-pyran-2-one + 6-benzyl-4-hydroxy-2H-pyran-2-one + 2-phenyl-1-(2,4,6-trihydroxyphenyl)ethanone + 3 CO2 | - |
? | |
3 malonyl-CoA + salicyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-2H-chromen-2-one + 3 CO2 | - |
? | |
4 malonyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
additional information | with acetyl CoA or malonyl CoA as a starter substrate, the enzyme only produces a triacetic lactone of triketide lactone type, with phenylacetyl-Coa also lactones of tetraketide and triketide type, and with hexanoyl-CoA phloroglucinol and triketide type lactones, substrate specificity, overview | Garcinia mangostana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
Synonyms | Comment | Organism |
---|---|---|
BPS | - |
Garcinia mangostana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant His-tagged BPS | Garcinia mangostana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
benzoyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
0.058 | - |
malonyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | recombinant His-tagged BPS | Garcinia mangostana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Garcinia mangostana | sequence calculation | - |
5.97 |
General Information | Comment | Organism |
---|---|---|
evolution | Garcinia mangostana BPS is an enzyme in the family of type III polyketide synthases and shows an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The three BPSs from the xanthone-rich Clusiaceae plants, Garcinia mangostana, Hypericum androsaemum, and Hypericum perforatum, suggest a close evolutionary relationship among these enzymes | Garcinia mangostana |
additional information | the catalytic triad is formed by Cys164, His303, and Asn336 | Garcinia mangostana |