BRENDA - Enzyme Database show
show all sequences of 2.3.1.151

Biosynthesis of biphenyls and benzophenones-evolution of benzoic acid-specific type III polyketide synthases in plants

Beerhues, L.; Liu, B.; Phytochemistry 70, 1719-1727 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Hypericum androsaemum
two cDNAs are isolated and heterologously expressed but both enzymes are inactive
Centaurium erythraea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0086
-
benzoyl-CoA
-
Hypericum androsaemum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42800
-
-
Hypericum androsaemum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Centaurium erythraea
-
-
-
Hypericum androsaemum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Centaurium erythraea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 3-hydroxybenzoyl-CoA
3-hydroxybenzoyl-CoA is the preferred substrate
706131
Centaurium erythraea
CoA + 2,3',4,6-tetrahydroxybenzophenone + CO2
-
-
-
?
Malonyl-CoA + benzoyl-CoA
enzyme is inactive with CoA-linked cinnamic acids
706131
Hypericum androsaemum
CoA + 2,4,6-trihydroxybenzophenone + CO2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Hypericum androsaemum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.055
-
benzoyl-CoA
-
Hypericum androsaemum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
7
-
Hypericum androsaemum
Cloned(Commentary) (protein specific)
Commentary
Organism
two cDNAs are isolated and heterologously expressed but both enzymes are inactive
Centaurium erythraea
expressed in Escherichia coli
Hypericum androsaemum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0086
-
benzoyl-CoA
-
Hypericum androsaemum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42800
-
-
Hypericum androsaemum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Centaurium erythraea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + 3-hydroxybenzoyl-CoA
3-hydroxybenzoyl-CoA is the preferred substrate
706131
Centaurium erythraea
CoA + 2,3',4,6-tetrahydroxybenzophenone + CO2
-
-
-
?
Malonyl-CoA + benzoyl-CoA
enzyme is inactive with CoA-linked cinnamic acids
706131
Hypericum androsaemum
CoA + 2,4,6-trihydroxybenzophenone + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Hypericum androsaemum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.055
-
benzoyl-CoA
-
Hypericum androsaemum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
7
-
Hypericum androsaemum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.414
-
benzoyl-CoA
-
Hypericum androsaemum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.414
-
benzoyl-CoA
-
Hypericum androsaemum
Other publictions for EC 2.3.1.151
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720639
Nualkaew
Benzophenone synthase from Gar ...
Garcinia mangostana
Phytochemistry
77
60-69
2012
-
-
1
-
4
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2
-
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3
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3
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1
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3
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9
1
1
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2
1
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1
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1
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4
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3
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1
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3
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9
1
1
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2
1
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1
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2
2
-
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-
704562
Klundt
A single amino acid substituti ...
Hypericum androsaemum
J. Biol. Chem.
284
30957-30964
2009
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-
1
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9
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4
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1
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2
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1
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3
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1
1
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2
2
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1
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9
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4
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1
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1
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3
-
1
1
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2
2
-
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-
-
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-
2
2
706131
Beerhues
Biosynthesis of biphenyls and ...
Centaurium erythraea, Hypericum androsaemum
Phytochemistry
70
1719-1727
2009
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-
2
-
-
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-
1
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1
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-
9
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1
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2
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1
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1
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1
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2
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1
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1
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1
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2
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1
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1
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1
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1
1
676362
Klingauf
Biosynthesis of the hyperforin ...
Hypericum calycinum
Phytochemistry
66
139-145
2005
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-
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-
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3
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1
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1
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660170
Liu
Benzophenone synthase and chal ...
Hypericum androsaemum
Plant J.
34
847-855
2003
1
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1
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2
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1
2
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3
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1
1
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1
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7
1
1
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1
1
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1
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1
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2
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1
2
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1
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1
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7
1
1
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1
1
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16327
Schmidt
Alternative pathways of xantho ...
Hypericum androsaemum
FEBS Lett.
420
143-146
1997
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3
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3
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1
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1
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3
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1
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1
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16326
Beerhues
Benzophenone synthase from cul ...
Centaurium erythraea
FEBS Lett.
383
264-266
1996
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1
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2
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1
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3
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1
1
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1
1
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1
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1
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3
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1
1
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1
1
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