Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dioleoyl phosphatidic acid | very strong activation | Umbelopsis ramanniana | |
dioleoyl phosphatidylserine | best phospholipid for activation | Umbelopsis ramanniana | |
dithiothreitol | 1 mM, 82% activation | Umbelopsis ramanniana | |
Phospholipids | required for activity | Umbelopsis ramanniana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitro-benzoate) | 1 mM, 77% inhibition | Umbelopsis ramanniana | |
Ca2+ | 5 mM, 70% inhibition | Umbelopsis ramanniana | |
EDTA | 1 mM, 79% inhibition | Umbelopsis ramanniana | |
Mg2+ | 5 mM, 75% inhibition | Umbelopsis ramanniana | |
N-ethylmaleimide | 5 mM, 79% inhibition | Umbelopsis ramanniana | |
p-chloromercuribenzoate | 1 mM, 64% inhibition | Umbelopsis ramanniana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
oleoyl-CoA | - |
Umbelopsis ramanniana | |
0.025 | - |
glycerol 3-phosphate | - |
Umbelopsis ramanniana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Umbelopsis ramanniana | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 2.5 mM, 53% activation, 10 mM, 100% activation | Umbelopsis ramanniana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SS-PAGE | Umbelopsis ramanniana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Umbelopsis ramanniana | - |
var. angulispora | - |
Purification (Comment) | Organism |
---|---|
heparin-Sepharose, Yellow 86-Sepharose, Superdex 200, hydroxylapatite | Umbelopsis ramanniana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.45 | - |
- |
Umbelopsis ramanniana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Umbelopsis ramanniana | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
myristoyl-CoA + sn-glycerol 3-phosphate | 20% of activity with oleoyl-CoA | Umbelopsis ramanniana | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
n-dodecanoyl-CoA + sn-glycerol 3-phosphate | very low activity | Umbelopsis ramanniana | CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate | - |
? | |
octanoyl-CoA + sn-glycerol 3-phosphate | very low activity | Umbelopsis ramanniana | CoA + 1-n-octanoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | best acyl-donor | Umbelopsis ramanniana | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | 20% of activity with oleoyl-CoA | Umbelopsis ramanniana | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | 20% of activity with oleoyl-CoA | Umbelopsis ramanniana | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SS-PAGE | Umbelopsis ramanniana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Umbelopsis ramanniana |