Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | activation of reconstituted mitochondrial enzyme | Rattus norvegicus | |
Lubrol Px | above the critical micellar concentration | Rattus norvegicus | |
phosphatidylcholine | i.e. lecithin, activation of mitochondrial enzyme | Rattus norvegicus | |
phosphatidylethanolamine | activation of reconstituted mitochondrial acyl transferase | Rattus norvegicus | |
phosphatidylglycerol | highest activation of reconstituted mitochondrial acyltransferase in the presence of Lubrol PX | Rattus norvegicus | |
phosphatidylinositol | activation of mitochondrial enzyme | Rattus norvegicus | |
phosphatidylserine | activation of mitochondrial enzyme | Rattus norvegicus | |
Zwittergent 3-14 | above the critical micellar concentration | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid | trivial name CHAPS, complete inhibition | Rattus norvegicus | |
cholate | complete inhibition | Rattus norvegicus | |
deoxycholate | complete inhibition | Rattus norvegicus | |
diacylglycerophosphate | - |
Rattus norvegicus | |
Monoacylglycerophosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
palmitoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol | Rattus norvegicus | |
0.006 | - |
palmitoyl-CoA | mitochondrial acyl transferase reconstituted in asolectin | Rattus norvegicus | |
0.0064 | - |
stearoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol | Rattus norvegicus | |
0.007 | - |
palmitoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol | Rattus norvegicus | |
0.0084 | - |
oleoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol | Rattus norvegicus | |
0.011 | - |
stearoyl-CoA | mitochondrial acyl transferase reconstituted in asolectin | Rattus norvegicus | |
0.012 | - |
myristoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol | Rattus norvegicus | |
0.013 | - |
stearoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol | Rattus norvegicus | |
0.013 | - |
oleoyl-CoA | mitochondrial acyl transferase reconstituted in asolectin | Rattus norvegicus | |
0.015 | - |
oleoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol | Rattus norvegicus | |
0.016 | - |
myristoyl-CoA | mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol | Rattus norvegicus | |
0.016 | - |
myristoyl-CoA | mitochondrial acyl transferase reconstituted in asolectin | Rattus norvegicus | |
0.46 | - |
glycerol 3-phosphate | mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol | Rattus norvegicus | |
0.82 | - |
glycerol 3-phosphate | mitochondrial acyl transferase reconstituted in asolectin | Rattus norvegicus | |
0.93 | - |
glycerol 3-phosphate | mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
1 * 85000, mitochondrial acyltransferase, SDS-PAGE | Rattus norvegicus |
95000 | 105000 | mitochondrial acyltransferase, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300 | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution in asolection or phosphatidylglycerol/phosphatidylethanolamine mixture | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.043 | - |
mitochondrial acyltransferase reconstituted in asolectin | Rattus norvegicus |
0.19 | - |
mitochondrial acyltransferase reconstituted in phosphatidylglycerol/phosphatidylethanolamine mixture | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, mitochondrial acyltransferase, SDS-PAGE | Rattus norvegicus |