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Literature summary for 2.3.1.145 extracted from
- The biosynthesis of piperine in Piper nigrum (1990), Phytochemistry, 29, 489-492.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 10 mM 2-mercaptoethanol stabilizes | Piper nigrum |
| preparations are to be carried out at 0-4°C | Piper nigrum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
piperoyl-CoA | - |
Piper nigrum |  |
| 10 | - |
piperidine | - |
Piper nigrum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-piperoyl-CoA + piperidine | Piper nigrum | - |
CoA + N-[(E,E)-piperoyl]-piperidine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Piper nigrum | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| cell homogenate has to be supplemented with 50 mM ascorbic acid as antioxidant | Piper nigrum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Piper nigrum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | - |
Piper nigrum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, enzyme stored as ammonium sulfate paste loses 20-25% of activity within one week, completely inactive after two weeks | Piper nigrum |
| diluted enzyme stored after anion-exchange chromatography is unstable | Piper nigrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-piperoyl-CoA + piperidine | - |
Piper nigrum | CoA + N-[(E,E)-piperoyl]-piperidine | - |
? | |
| piperoyl-CoA + 3-pyrroline | 25% of activity with piperidine | Piper nigrum | CoA + piperoyl-(3-pyrroline) | - |
? | |
| piperoyl-CoA + pyrrolidine | 76% of activity with piperidine | Piper nigrum | CoA + piperoylpyrrolidine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Piper nigrum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 42 | heat-denaturation | Piper nigrum |
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Release 2023.1 (January 2023)
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