Literature summary for 2.3.1.144 extracted from

Eudes, A.; Baidoo, E.E.; Yang, F.; Burd, H.; Hadi, M.Z.; Collins, F.W.; Keasling, J.D.; Loque, D.
Production of tranilast [N-(3,4-dimethoxycinnamoyl)-anthranilic acid] and its analogs in yeast Saccharomyces cerevisiae (2011), Appl. Microbiol. Biotechnol., 89, 989-1000.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of HCBT in Saccharomyces cerevisiae, coexpression with 4-coumarate/CoA ligase from Arabidopsis thaliana using yeast shuttle vector pDRf1-GW-PHXT7	Dianthus caryophyllus

Protein Variants

Protein Variants	Comment	Organism
additional information	for production of tranilast, i.e. N-(3,4-dimethoxycinnamoyl)-anthranilate, and several of its analogues, a yeast strain is engineered to coexpress a 4-coumarate/CoA ligase, EC 6.2.1.12, from Arabidopsis thaliana and the hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase from Dianthus caryophyllus. Production of tranilast and 26 different cinnamoyl anthranilate molecules within a few hours after exogenous supply of various combinations of cinnamic acids and anthranilate derivatives, method optimization, overview	Dianthus caryophyllus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
coumaroyl-CoA + anthranilate	Dianthus caryophyllus	-	CoA + N-(4-hydroxycinnamoyl)-anthranilate	product detection by mass spectrometry	?

Organism

Organism	UniProt	Comment	Textmining
Dianthus caryophyllus	O24645	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2,3-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(2,3-dimethoxycinnamoyl)-3-hydroxyanthranilate	-	?
2,3-dimethoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(2,3-dimethoxycinnamoyl)-anthranilate	-	?
2,4-dimethoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(2,4-dimethoxycinnamoyl)-anthranilate	-	?
2,5-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(2,5-dimethoxycinnamoyl)-3-hydroxyanthranilate	-	?
2,5-dimethoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(2,5-dimethoxycinnamoyl)-anthranilate	-	?
2-coumaroyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(2-coumaroyl)-3-hydroxyanthranilate	-	?
2-coumaroyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(2-coumaroyl)anthranilate	-	?
3,4,5-trimethoxycinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(3,4,5-trimethoxycinnamoyl)-3-hydroxyanthranilate	-	?
3,4,5-trimethoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(3,4,5-trimethoxycinnamoyl)-anthranilate	-	?
3,4-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(3,4-dimethoxycinnamoyl)-3-hydroxyanthranilate	-	?
3-coumaroyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(3-coumaroyl)-3-hydroxyanthranilate	-	?
3-coumaroyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(3-hydroxycinnamoyl)-anthranilate	-	?
3-hydroxy-4-methoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(3-hydroxy-4-methoxycinnamoyl)-anthranilate	-	?
3-methoxycinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(3-methoxycinnamoyl)-3-hydroxyanthranilate	-	?
3-methoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(3-methoxycinnamoyl)-anthranilate	-	?
4-coumaroyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-(4-coumaroyl)-3-hydroxyanthranilate	-	?
4-coumaroyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(4-coumaroyl)-anthranilate	product detection by mass spectrometry	?
4-methoxycinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(4-methoxycinnamoyl)-anthranilate	-	?
caffeoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-caffeoyl-3-hydroxyanthranilate	-	?
caffeoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(3,4-dihydroxycinnamoyl)-anthranilate	-	?
cinnamoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-cinnamoyl-3-hydroxyanthranilate	-	?
cinnamoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-cinnamoylanthranilate	-	?
coumaroyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-(4-hydroxycinnamoyl)-anthranilate	product detection by mass spectrometry	?
feruloyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-feruloyl-3-hydroxyanthranilate	-	?
feruloyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-feruloylanthranilate	-	?
additional information	hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase, HCBT, has affinity for anthranilate and 4-coumaroyl-CoA and is capable of producing N-(4-hydroxycinnamoyl)-anthranilate in vitro	Dianthus caryophyllus	?	-	?
sinapoyl-CoA + 3-hydroxyanthranilate	-	Dianthus caryophyllus	CoA + N-sinapoyl-3-hydroxyanthranilate	-	?
sinapoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-sinapoylanthranilate	-	?

Synonyms

Synonyms	Comment	Organism
benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase	-	Dianthus caryophyllus
HCBT	-	Dianthus caryophyllus
hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase	-	Dianthus caryophyllus