Cloned (Comment) | Organism |
---|---|
expression of HCBT in Saccharomyces cerevisiae, coexpression with 4-coumarate/CoA ligase from Arabidopsis thaliana using yeast shuttle vector pDRf1-GW-PHXT7 | Dianthus caryophyllus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | for production of tranilast, i.e. N-(3,4-dimethoxycinnamoyl)-anthranilate, and several of its analogues, a yeast strain is engineered to coexpress a 4-coumarate/CoA ligase, EC 6.2.1.12, from Arabidopsis thaliana and the hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase from Dianthus caryophyllus. Production of tranilast and 26 different cinnamoyl anthranilate molecules within a few hours after exogenous supply of various combinations of cinnamic acids and anthranilate derivatives, method optimization, overview | Dianthus caryophyllus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coumaroyl-CoA + anthranilate | Dianthus caryophyllus | - |
CoA + N-(4-hydroxycinnamoyl)-anthranilate | product detection by mass spectrometry | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | O24645 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(2,3-dimethoxycinnamoyl)-3-hydroxyanthranilate | - |
? | |
2,3-dimethoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(2,3-dimethoxycinnamoyl)-anthranilate | - |
? | |
2,4-dimethoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(2,4-dimethoxycinnamoyl)-anthranilate | - |
? | |
2,5-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(2,5-dimethoxycinnamoyl)-3-hydroxyanthranilate | - |
? | |
2,5-dimethoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(2,5-dimethoxycinnamoyl)-anthranilate | - |
? | |
2-coumaroyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(2-coumaroyl)-3-hydroxyanthranilate | - |
? | |
2-coumaroyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(2-coumaroyl)anthranilate | - |
? | |
3,4,5-trimethoxycinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(3,4,5-trimethoxycinnamoyl)-3-hydroxyanthranilate | - |
? | |
3,4,5-trimethoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(3,4,5-trimethoxycinnamoyl)-anthranilate | - |
? | |
3,4-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(3,4-dimethoxycinnamoyl)-3-hydroxyanthranilate | - |
? | |
3-coumaroyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(3-coumaroyl)-3-hydroxyanthranilate | - |
? | |
3-coumaroyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(3-hydroxycinnamoyl)-anthranilate | - |
? | |
3-hydroxy-4-methoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(3-hydroxy-4-methoxycinnamoyl)-anthranilate | - |
? | |
3-methoxycinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(3-methoxycinnamoyl)-3-hydroxyanthranilate | - |
? | |
3-methoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(3-methoxycinnamoyl)-anthranilate | - |
? | |
4-coumaroyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-(4-coumaroyl)-3-hydroxyanthranilate | - |
? | |
4-coumaroyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(4-coumaroyl)-anthranilate | product detection by mass spectrometry | ? | |
4-methoxycinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(4-methoxycinnamoyl)-anthranilate | - |
? | |
caffeoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-caffeoyl-3-hydroxyanthranilate | - |
? | |
caffeoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(3,4-dihydroxycinnamoyl)-anthranilate | - |
? | |
cinnamoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-cinnamoyl-3-hydroxyanthranilate | - |
? | |
cinnamoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-cinnamoylanthranilate | - |
? | |
coumaroyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-(4-hydroxycinnamoyl)-anthranilate | product detection by mass spectrometry | ? | |
feruloyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-feruloyl-3-hydroxyanthranilate | - |
? | |
feruloyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-feruloylanthranilate | - |
? | |
additional information | hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase, HCBT, has affinity for anthranilate and 4-coumaroyl-CoA and is capable of producing N-(4-hydroxycinnamoyl)-anthranilate in vitro | Dianthus caryophyllus | ? | - |
? | |
sinapoyl-CoA + 3-hydroxyanthranilate | - |
Dianthus caryophyllus | CoA + N-sinapoyl-3-hydroxyanthranilate | - |
? | |
sinapoyl-CoA + anthranilate | - |
Dianthus caryophyllus | CoA + N-sinapoylanthranilate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase | - |
Dianthus caryophyllus |
HCBT | - |
Dianthus caryophyllus |
hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase | - |
Dianthus caryophyllus |