Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.142 extracted from

  • Baghel, A.S.; Tandon, R.; Gupta, G.; Kumar, A.; Sharma, R.K.; Aggarwal, N.; Kathuria, A.; Saini, N.K.; Bose, M.; Prasad, A.K.; Sharma, S.K.; Nath, M.; Parmar, V.S.; Raj, H.G.
    Characterization of protein acyltransferase function of recombinant purified GlnA1 from Mycobacterium tuberculosis: a moon lighting property (2011), Microbiol. Res., 166, 662-672.
    View publication on PubMed
Search for more literature for 2.3.1.142

Cloned(Commentary)

Cloned (Comment) Organism
gene glnA1 or Rv2220, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
additional information L-methionine-S-sulfoximine, a potent inhibitor of GlnA1, and a structural analogue of glutamate, fails to inhibit TAase activity of rGlnA1 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 7,8-diacetoxy-4-methylcoumarin pH 6.5, 37°C, recombinant GlnA1 Mycobacterium tuberculosis
0.188
-
 acetyl-CoA pH 6.5, 37°C, recombinant GlnA1 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted protein Mycobacterium tuberculosis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione-S-transferase + 7,8-diacetoxy-4-methylcoumarin Mycobacterium tuberculosis
-
 ?
-
 ?
additional information Mycobacterium tuberculosis besides glutamine synthetase activity, recombinant glutamine synthetase, GlnA1, of Mycobacterium tuberculosis shows additional MTAase function. MTAase exhibits differential specificities to several acyloxycoumarins, and is also capable of transferring propionyl and butyryl groups from propoxy and butoxy derivatives of 4-methylcoumarin, detailed overview. MTAase fails to accept as substrates the compounds bearing acetoxy group on the lactone ring at C-3/C-4 position of coumarin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 glnA1 gene, Rv2220
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione-S-transferase + 7,8-diacetoxy-4-methylcoumarin
-
 Mycobacterium tuberculosis ?
-
 ?
glutathione-S-transferase + acetyl-CoA
-
 Mycobacterium tuberculosis ?
-
 ?
additional information besides glutamine synthetase activity, recombinant glutamine synthetase, GlnA1, of Mycobacterium tuberculosis shows additional MTAase function. MTAase exhibits differential specificities to several acyloxycoumarins, and is also capable of transferring propionyl and butyryl groups from propoxy and butoxy derivatives of 4-methylcoumarin, detailed overview. MTAase fails to accept as substrates the compounds bearing acetoxy group on the lactone ring at C-3/C-4 position of coumarin Mycobacterium tuberculosis ?
-
 ?

Synonyms

Synonyms Comment Organism
GlnA1
-
 Mycobacterium tuberculosis
MTAase
-
 Mycobacterium tuberculosis
protein acetyltransferase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Mycobacterium tuberculosis

General Information

General Information Comment Organism
additional information active site of rGlnA1 for TAase activity, overview Mycobacterium tuberculosis
physiological function GlnA1 is a virulence determinant factor. The extracellular secretion of GlnA1 in large amounts is thought to play an important role in the biosynthesis of poly L-glutamate/glutamine cell wall Mycobacterium tuberculosis