Cloned (Comment) | Organism |
---|---|
expression of wild-type CyaC acyltransferase and various mutants in Escherichia coli | Bordetella pertussis |
Protein Variants | Comment | Organism |
---|---|---|
A140G | changed selectivity of acyltransferase for the 2 acetylation sites of adenylate cyclase toxin, a mixture of bi and monoacetylated toxins modified either at both Lys860 and Lys983 or only at Lys860 is generated | Bordetella pertussis |
A140V | monoacetylation of adenylate cyclase toxin almost exclusively at Lys983 | Bordetella pertussis |
C67S | no effect on adenylate cyclase toxin activation | Bordetella pertussis |
H33D | loss of adenlayte cyclase toxin acetylation activity | Bordetella pertussis |
H33S | loss of adenlayte cyclase toxin acetylation activity | Bordetella pertussis |
R141K | no effect on adenylate cyclase toxin activation | Bordetella pertussis |
R141L | no effect on adenylate cyclase toxin activation | Bordetella pertussis |
S30R | loss of adenlayte cyclase toxin acetylation activity | Bordetella pertussis |
S30W | loss of adenlayte cyclase toxin acetylation activity | Bordetella pertussis |
S68T | no effect on adenylate cyclase toxin activation | Bordetella pertussis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + adenylate cyclase toxin | Bordetella pertussis | palmitoylation activates adenylate cyclase toxin | CoA + palmitoyladenylate cyclase toxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella pertussis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + adenylate cyclase toxin | acyltransferase CyaC palmitoylates the conserved lysines 983 and 860 of adenylate cyclase toxin | Bordetella pertussis | CoA + palmitoyladenylate cyclase toxin | - |
? | |
palmitoyl-CoA + adenylate cyclase toxin | palmitoylation activates adenylate cyclase toxin | Bordetella pertussis | CoA + palmitoyladenylate cyclase toxin | - |
? |