Any feedback?
Literature summary for 2.3.1.136 extracted from
- Biochemical characterization of the polysialic acid-specific O-acetyltransferase NeuO of Escherichia coli K1 (2007), J. Biol. Chem., 282, 22217-22227.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a C-terminal hexahistidine-tagged fusion protein | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H119A | complete loss of enzymatic activity without affecting the oligomerization state | Escherichia coli |
| NeuO + 12 hepta-nucleotide repeats | a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 3.7 mg/L, Km (mM): 0.22 (acetyl-CoA), 1.1 (colomnic acid), kcat (1/sec): 0.56 (acetyl-CoA), 1.85 (colomnic acid) | Escherichia coli |
| NeuO + 24 hepta-nucleotide repeats | a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.27 (acetyl-CoA), 1.0 (colomnic acid), kcat (1/sec): 1.07 (acetyl-CoA), 2.57 (colomnic acid) | Escherichia coli |
| NeuO + 36 hepta-nucleotide repeats | a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.22 (acetyl-CoA), 0.22 (propionyl-CoA) 1.6 (colomnic acid), kcat (1/sec): 1.16 (acetyl-CoA), 0.09 (propionyl-CoA), 3.84 (colomnic acid) | Escherichia coli |
| W143A | complete loss of enzymatic activity without affecting the oligomerization state | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
acetyl-CoA | mutant NeuO + 12 hepta-nucleotide repeats | Escherichia coli |  |
| 0.22 | - |
acetyl-CoA | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
| 0.22 | - |
propionyl-CoA | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
| 0.27 | - |
acetyl-CoA | mutant NeuO + 24 hepta-nucleotide repeats | Escherichia coli | |
| 0.4 | - |
acetyl-CoA | wild-type NeuO | Escherichia coli | |
| 1 | - |
colominic acid | mutant NeuO + 24 hepta-nucleotide repeats | Escherichia coli | |
| 1.06 | - |
colominic acid | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
| 1.1 | - |
colominic acid | mutant NeuO + 12 hepta-nucleotide repeats | Escherichia coli | |
| 1.17 | - |
colominic acid | wild-type NeuO | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26800 | - |
SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni2+-affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzymatic activity increases linearly with increasing numbers of repeats | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + (-8-Neu5Ac-alpha2)2 | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + (-8-Neu5Ac-alpha2)3 | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + (-8-Neu5Ac-alpha2)4 | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + (-8-Neu5Ac-alpha2)5 | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + CMP-Neu5Ac | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + colominic acid | - |
Escherichia coli | CoA + O-(acetyl)-colominic acid | - |
? | |
| acetyl-CoA + Neu5Ac | - |
Escherichia coli | CoA + O-(acetyl)-Neu5Ac | - |
? | |
| additional information | enzyme shows a high substrate specificity toward polysialic acid with more than 14 residues | Escherichia coli | ? | - |
? | |
| propionyl-CoA + colominic acid | - |
Escherichia coli | CoA + O-(propionyl)-colominic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | gel filtration | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NeuO | - |
Escherichia coli |
| polysialic acid-specific O-acetyltransferase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
propionyl-CoA | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
| 0.51 | - |
acetyl-CoA | wild-type NeuO | Escherichia coli | |
| 0.56 | - |
acetyl-CoA | mutant NeuO + 12 hepta-nucleotide repeats | Escherichia coli | |
| 1.07 | - |
acetyl-CoA | mutant NeuO + 24 hepta-nucleotide repeats | Escherichia coli | |
| 1.16 | - |
acetyl-CoA | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
| 1.85 | - |
colominic acid | mutant NeuO + 12 hepta-nucleotide repeats | Escherichia coli | |
| 1.99 | - |
colominic acid | wild-type NeuO | Escherichia coli | |
| 2.57 | - |
colominic acid | mutant NeuO + 24 hepta-nucleotide repeats | Escherichia coli | |
| 3.84 | - |
colominic acid | mutant NeuO + 36 hepta-nucleotide repeats | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
