General Stability | Organism |
---|---|
endogenous polysialic acid stabilizes by a close non-covalent association with the enzyme | Escherichia coli |
Triton X-100, 0.05%, stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
acetyl-CoA | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | eventually membrane-bound or membrane-associated protein | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of a divalent cation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K1 OAc+ substrains | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, at least 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + colominic acid | substrate is a commercially available mixture of polysialic acid fragments, about 15 sialic acid residues per polymer, polysialic acids with more than 14 residues are acylated | Escherichia coli | CoA + O-(acetyl)-colominic acid | product is polysialic acid 7- or 9-O-acetylated | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Escherichia coli |