Literature summary for 2.3.1.135 extracted from

Horchani, H.; Bussi�res, S.; Cantin, L.; Lhor, M.; Lalibert�-Gemme, J.S.; Breton, R.; Salesse C.
Enzymatic activity of lecithin:retinol acyltransferase: a thermostable and highly active enzyme with a likely mode of interfacial activation (2014), Biochim. Biophys. Acta, 1844, 1128-1836.

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Activating Compound

Activating Compound	Comment	Organism	Structure
SDS	SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: retinyl heptanoate	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs	Homo sapiens
0.055	-	all-trans-retinol	pH not specified in the publication, 20°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O95237	-	-

Purification (Commentary)

Purification (Comment)	Organism
SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability. Detergents such as 0.2% Triton X-100, 0.7% CHAPSO,and 1.2 mM n-dodecyl-beta-D-maltoside and sodium are not effective	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
didecanoylphosphatidylcholine + all-trans-retinol	-	Homo sapiens	all-trans-retinyl decanoate + 2-decanoylglycerophosphocholine	activity is about 10% of that with diheptanoylphosphatidylcholine	?
diheptanoylphosphatidylcholine + all-trans-retinol	-	Homo sapiens	all-trans-retinyl heptanoate + 2-heptanoylglycerophosphocholine	-	?
dilauroylphosphatidylcholine + all-trans-retinol	-	Homo sapiens	all-trans-retinyl laurate + 2-lauroylglycerophosphocholine	-	?
dilauroylphosphatidylcholine + all-trans-retinol	-	Homo sapiens	all-trans-retinyl laurate + 2-lauroylglycerophosphocholine	activity is about 4% of that with diheptanoylphosphatidylcholine	?
dimyristoylphosphatidylcholine + all-trans-retinol	activity is about 3% of that with diheptanoylphosphatidylcholine	Homo sapiens	all-trans-retinyl myristate + 2-myristoylglycerophosphocholine	-	?
dioctanoylphosphatidylcholine + all-trans-retinol	activity is less than 15% of that with diheptanoylphosphatidylcholine	Homo sapiens	all-trans-retinyl octanoate + 2-octanoylglycerophosphocholine	-	?
dioleoylphosphatidylcholine + all-trans-retinol	-	Homo sapiens	all-trans-retinyl oleate + 2-oleoylglycerophosphocholine	-	?
dipalmitoylphosphatidylcholine + all-trans-retinol	activity is about 2% of that with diheptanoylphosphatidylcholine	Homo sapiens	all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine	-	?

Synonyms

Synonyms	Comment	Organism
lecithin:retinol acyltransferase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	25	-	Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	-	15% of maximum activity	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
40.4	-	all-trans-retinol	pH not specified in the publication, 20°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.2	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
10	-	80% of maximum activity	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
730	-	all-trans-retinol	pH not specified in the publication, 20°C	Homo sapiens

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Release 2023.1 (January 2023)