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Literature summary for 2.3.1.135 extracted from
- Role of LRAT on the retinoid isomerase activity and membrane association of Rpe65 (2007), J. Biol. Chem., 282, 20915-20924.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable expression in HEK-293T cells | Mus musculus |
| stable expression in HEK-293T cells | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mus musculus |  |
| Ca2+ | - |
Bos taurus | |
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lecithin + retinol-[cellular retinol-binding protein] | Mus musculus | - |
2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| lecithin + retinol-[cellular retinol-binding protein] | Bos taurus | - |
2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| lecithin + retinol-[cellular retinol-binding protein] | Mus musculus 129S2/Sv | - |
2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| additional information | Mus musculus | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | ? | - |
? | |
| additional information | Bos taurus | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | ? | - |
? | |
| additional information | Mus musculus 129S2/Sv | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Mus musculus 129S2/Sv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by membrane preparation from retinal pigment epithelium | Mus musculus |
| native enzyme partially by membrane preparation from retinal pigment epithelium | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | - |
Mus musculus | - |
| eye | - |
Bos taurus | - |
| retinal pigment epithelium | - |
Mus musculus | - |
| retinal pigment epithelium | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lecithin + retinol-[cellular retinol-binding protein] | - |
Mus musculus | 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| lecithin + retinol-[cellular retinol-binding protein] | - |
Bos taurus | 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| lecithin + retinol-[cellular retinol-binding protein] | - |
Mus musculus 129S2/Sv | 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein] | - |
? | |
| additional information | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | Mus musculus | ? | - |
? | |
| additional information | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | Bos taurus | ? | - |
? | |
| additional information | no activity with Rpe65 protein | Mus musculus | ? | - |
? | |
| additional information | no activity with Rpe65 protein | Bos taurus | ? | - |
? | |
| additional information | LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview | Mus musculus 129S2/Sv | ? | - |
? | |
| additional information | no activity with Rpe65 protein | Mus musculus 129S2/Sv | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lecithin:retinol acyl transferase | - |
Mus musculus |
| lecithin:retinol acyl transferase | - |
Bos taurus |
| LRAT | - |
Mus musculus |
| LRAT | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
| 7.5 | - |
assay at | Bos taurus |
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