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Literature summary for 2.3.1.135 extracted from
- Roles of cysteine 161 and tyrosine 154 in the lecithin-retinol acyltransferase mechanism (2004), Biochemistry, 43, 6120-6126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K104A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| K133A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
| K133A/K134A | site-directed mutagenesis, slightly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
| K134A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
| K147A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| K180A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
| K180R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| K186A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
| K186R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| K90A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| K95A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
| Y118F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
| Y154F | site-directed mutagenesis, inactive mutant | Homo sapiens |
| Y167F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
| Y64F | site-directed mutagenesis, highly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | Homo sapiens | - |
2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95237 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphatidylcholine + retinol-[cellular-retinol-binding-protein] = 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | mechanism, Cys161 and Tyr154 are essential for activity | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of recombinant mutant enzymes and recombinant truncated wild-type, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + all-trans-retinol | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl acylester | - |
? | |
| phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? | |
| phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lecithin-retinol acyltransferase | - |
Homo sapiens |
| LRAT | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
about | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile of recombinant mutant enzymes and recombinant truncated wild-type | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
inactivation of recombinant truncated wild-type enzyme, reversible by dialysis against a buffer with pH 8.4 | Homo sapiens |
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