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Literature summary for 2.3.1.133 extracted from
- Down-regulation of hydroxycinnamoyl CoA: Shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality (2007), Phytochemistry, 68, 1521-1529.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | HCT is evaluated as a target for forage quality improvement | Medicago sativa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| an antisense construct is generated for down-regulation of HCT | Medicago sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + shikimate | Medicago sativa | - |
CoA + 5-O-(4-coumaroyl)shikimate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
Medicago sativa L. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| crude extracts from stem material from various down-regulated and control lines are assayed for extractable HCT enzyme activity | Medicago sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Medicago sativa | - |
| vascular tissue | - |
Medicago sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
reduction of enzyme activity in down-regulated lines is from at least 15 - 50% | Medicago sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + shikimate | - |
Medicago sativa | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase | - |
Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
HCT enzyme activity assay | Medicago sativa |
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