Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E227Q | inactive | Homo sapiens |
F168L | the mutant shows decreased activity compared to the wild type enzyme | Homo sapiens |
K16N | the mutant shows wild type activity | Homo sapiens |
N156S | the mutant shows increased activity compared to the wild type enzyme | Homo sapiens |
R131H | the mutant shows wild type activity | Homo sapiens |
R199C | inactive | Homo sapiens |
S17T | the mutant shows wild type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
benzoyl-CoA | mutant enzyme K16N, apparent value, at pH 8.0 and 37°C | Homo sapiens | |
0.024 | - |
benzoyl-CoA | wild type enzyme, apparent value, at pH 8.0 and 37°C | Homo sapiens | |
0.028 | - |
benzoyl-CoA | mutant enzyme S17T, apparent value, at pH 8.0 and 37°C | Homo sapiens | |
0.038 | - |
benzoyl-CoA | mutant enzyme N156S, apparent value, at pH 8.0 and 37°C | Homo sapiens | |
0.053 | - |
benzoyl-CoA | mutant enzyme F168L, apparent value, at pH 8.0 and 37°C | Homo sapiens | |
0.071 | - |
benzoyl-CoA | mutant enzyme R131H, apparent value, at pH 8.0 and 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6IB77 | - |
- |
Purification (Comment) | Organism |
---|---|
Protino Ni-TED 2000 column chromatography and Sephadex G25 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + glycine | - |
Homo sapiens | CoA + N-benzoylglycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GlyAT | - |
Homo sapiens |