Literature summary for 2.3.1.122 extracted from

Kreme, L.; Maughan, W.N.; Wilson, R.A.; Dover, L.G.; Besra, G.S.
The M. tuberculosis antigen 85 complex and mycolyltransferase activity (2002), Lett. Appl. Microbiol., 34, 233-237.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of FbpA, FbpB and FbpC2 in Escherichia coli, FbpB poorly expressed due to differential efficiency of mRNA translation	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
6-azido-6-deoxytrehalose	inhibits all three members of Ag85 complex in vitro	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	FbpA: SDS-PAGE, Western Blot, aminoacid sequence. FbpB: aminoacid sequence. FbpC2: SDS-PAGE, Western Blot, aminoacid sequence	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns	Mycobacterium tuberculosis

Reaction

Reaction	Comment	Organism	Reaction ID
2 alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate	reaction catalyzed by 3 members of the Ag85 complex in vitro, namely FbpA, FbpB and FbpC2	Mycobacterium tuberculosis	a

Subunits

Subunits	Comment	Organism
monomer	FbpA: 1 * 30000, SDS-PAGE. FbpC2: 1 * 30000, SDS-PAGE	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)