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Literature summary for 2.3.1.12 extracted from

  • Hendle, J.; Mattevi, A.; Westphal, A.H.; Spee, J.; de Kok, A.; Teplyakov, A.; Hol, W.G.
    Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipoamide acetyltransferase (E2p) (1995), Biochemistry, 34, 4287-4298.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Azotobacter vinelandii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Azotobacter vinelandii

Protein Variants

Protein Variants Comment Organism
G610C 500fold reduced enzymatic activity Azotobacter vinelandii
N614D 9fold reduced enzymatic activity Azotobacter vinelandii
S558A 200fold reduced enzymatic activity Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 acetyl-CoA wild-type enzyme Azotobacter vinelandii
0.028
-
 acetyl-CoA S558A mutant Azotobacter vinelandii
0.036
-
 acetyl-CoA H610C mutant Azotobacter vinelandii
0.052
-
 acetyl-CoA N614D mutant Azotobacter vinelandii
1.08
-
 dihydrolipoamide N614D mutant Azotobacter vinelandii
5.3
-
 dihydrolipoamide H610C mutant Azotobacter vinelandii
5.37
-
 dihydrolipoamide wild-type enzyme Azotobacter vinelandii
6.4
-
 dihydrolipoamide S558A mutant Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrolipoamide + acetyl-CoA Azotobacter vinelandii
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
catalytic domain mutants Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + acetyl-CoA
-
 Azotobacter vinelandii S-acetyldihydrolipoamide + CoA
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00417
-
 acetyl-CoA with dihydrolipoamide, H610C mutant Azotobacter vinelandii
0.0143
-
 acetyl-CoA with dihydrolipoamide, S558A mutant Azotobacter vinelandii
0.282
-
 acetyl-CoA with dihydrolipoamide, N614D mutant Azotobacter vinelandii
2.4
-
 acetyl-CoA with dihydrolipoamide, wild-type enzyme Azotobacter vinelandii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.4 stable Azotobacter vinelandii