Literature summary for 2.3.1.118 extracted from

Saito, K.; Shinohara, A.; Kamataki, T.; Kato, R.
Metabolic activation of mutagenic N-hydroxyarylamines by O-acetyltransferase in Salmonella typhimurium TA98 (1985), Arch. Biochem. Biophys., 239, 286-295.

Search for more literature for 2.3.1.118

Inhibitors

Inhibitors	Comment	Organism	Structure
1-Nitro-2-naphthol	-	Salmonella enterica subsp. enterica serovar Typhimurium
2-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	Salmonella enterica subsp. enterica serovar Typhimurium
coenzyme A	-	Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetamide	-	Salmonella enterica subsp. enterica serovar Typhimurium
additional information	no inhibition by 2,6-dichloro-4-nitrophenol, chloramphenicol, hydroxylamine; no inhibition by paraoxon	Salmonella enterica subsp. enterica serovar Typhimurium
N-ethylmaleimide	-	Salmonella enterica subsp. enterica serovar Typhimurium
p-chloromercuribenzoate	-	Salmonella enterica subsp. enterica serovar Typhimurium
Pentachlorophenol	-	Salmonella enterica subsp. enterica serovar Typhimurium
thiolactomycin	-	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0033	-	acetyl-CoA	reaction with O-acetyl transfer to 2-hydroxyamino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole as substrate	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + an N-hydroxyarylamine	Salmonella enterica subsp. enterica serovar Typhimurium	involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species	CoA + an N-acetoxyarylamine	-	?
acetyl-CoA + an N-hydroxyarylamine	Salmonella enterica subsp. enterica serovar Typhimurium	the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates	CoA + an N-acetoxyarylamine	-	?
acetyl-CoA + an N-hydroxyarylamine	Salmonella enterica subsp. enterica serovar Typhimurium	unknown endogenous substrate in bacteria	CoA + an N-acetoxyarylamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium TA98	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial, by ammonium sulfate precipitation, DEAE-cellulose and Sephadex-G150 column chromatography	Salmonella enterica subsp. enterica serovar Typhimurium
streptomycin, ammonium sulfate precipitation, DEAE Cellulose, Sephadex G-150 chromatograpy	Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.126	-	2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate	Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability	Organism
-80°C, at least 3 months	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	N-hydroxy-2-acetylaminofluorene cannot replace acetyl-CoA	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	N-hydroxy-2-acetylaminofluorene cannot replace acetyl-CoA	Salmonella enterica subsp. enterica serovar Typhimurium TA98	CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
acetyl-CoA + 3-hydroxyamino-1-methyl-pyrido[4,3-b]-indole	-	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-acetoxy-amino-1-methyl-pyrido[4,3-b]-indole	-	?
acetyl-CoA + an N-hydroxyarylamine	involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + an N-acetoxyarylamine	-	?
acetyl-CoA + an N-hydroxyarylamine	the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + an N-acetoxyarylamine	-	?
acetyl-CoA + an N-hydroxyarylamine	unknown endogenous substrate in bacteria	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + an N-acetoxyarylamine	-	?
acetyl-CoA + N-hydroxy-2-aminofluorene	-	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-acetoxy-2-aminofluorene	-	?
butyryl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	11% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-(butyryloxy)-6-methylpyrido[3',2':4,5]imidazo[1,2-a]pyridin-2-amine	-	?
butyryl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	11% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium TA98	CoA + N-(butyryloxy)-6-methylpyrido[3',2':4,5]imidazo[1,2-a]pyridin-2-amine	-	?
hexanoyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	1.3% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-hexanoyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
malonyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	19% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-malonyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
malonyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	19% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium TA98	CoA + N-malonyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
additional information	the N-acetoxyarylamine products form covalent adducts with cellular macromolecules, e.g. tRNA	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?
additional information	the N-acetoxyarylamine products form covalent adducts with cellular macromolecules, e.g. tRNA	Salmonella enterica subsp. enterica serovar Typhimurium TA98	?	-	?
propionyl-CoA + 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	89% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + N-propionyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?
propionyl-CoA + 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	89% of the activity compared to acetyl-CoA as acyl donor	Salmonella enterica subsp. enterica serovar Typhimurium TA98	CoA + N-propionyloxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole	-	?

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Release 2023.1 (January 2023)